Core F(B): Synthesis Design

脂质合成核心(核心F)将通过提供合成化学来服务于脂质MAP联盟 对脂质核心群体的支持。核心F由两个子核心组成：脂质标准F(A)和 综合设计，F(B)。脂质标准子核心由Avanti Polar Lipids，Inc.代表。 CORE将作为脂质地图联盟的制造部门，因此有责任 (A)设计和执行由财团发现的新脂质的合成路线，(B)生产新的 激动剂，如Kdo2-Lipid A，(C)产生可用作内部标准的脂类产品，如 稳定同位素标记化合物和含有单链脂肪酸的化合物，(D)产生脂类产品 可用作主要的质谱学标准，如天然产物的合成类似物， (E)为具有规定浓度和最长保质期的质谱学标准制定包装， (F)开发核心F(B)开发的低密度脂蛋白氧化类脂模拟物的生产规模合成，以及(G) 继续对上一批赠款期间开发的产品进行生产和稳定性测试。所有的 制造和表征的化合物将免费提供给财团成员， 而更大的脂类社区将通过Avanti目录获得这些产品。 合成设计子核心将为新的脂类化合物开发合成途径，包括 氧化型低密度脂蛋白和单碳链脂肪酸中存在不同程度不饱和的活化剂。 核心F(B)将与脂肪标准分核心密切合作，发展生产规模制造 这些化合物的工艺。 核心F拟议的具体目标的成功完成将确保脂体核心 集团拥有纯净、稳定的化合物，这些化合物对于完成 财团。从本质上讲，Core F提供的脂类标准是研究的基础 由其他核心实验室提出的建议是基于。

The Lipid Synthesis Core (Core F) will serve the LIPID MAPS Consortium by providing synthetic chemistry support for the Lipidomic Core groups. Core F is composed of two sub-Cores: Lipid Standards, F(A) and Synthesis Design, F(B). The Lipid Standards sub-Core is represented by Avanti Polar Lipids, Inc. This sub- Core will serve as the manufacturing arm of the LIPID MAPS consortium, and as such has the responsibility to (a) design and execute synthetic routes to novel lipids discovered by the consortium, (b) produce novel agonists, such as Kdo2-Lipid A, (c) produce lipid products that can be used as internal standards, such as stable isotope-labeled compounds and compounds containing odd chain fatty acids, (d) produce lipid products that can be used as primary mass spectrometric standards, such as synthetic analogues of natural products, (e) develop packaging for mass spectrometric standards with defined concentrations and maximum shelf life, (f) develop production scale synthesis of the LDL oxidized lipid mimetic developed by Core F(B), and (g) continue the production and stability testing of products developed in the previous grant period. All of the compounds manufactured and characterized will be made available to the consortium members at no charge, while the larger lipid community will have access to these products through the Avanti catalog. The Synthesis Design sub-Core will develop synthetic pathways for novel lipid compounds including the active agent present in oxidized LDL and odd-carbon chain fatty acids with varying degrees of unsaturation. Core F(B) will work closely with the Lipid Standards sub-Core to develop production scale manufacturing processes for these compounds. The successful completion of the proposed specific aims of Core F will ensure that the Lipidomic Core groups have pure, stable compounds, which are essential to the completion of the overall objectives of the Consortium. In essence, the lipid standards provided by Core F are the foundations on which the research proposed by the other Core laboratories is based.