PROTEIN X-RAY CRYSTALLOGRAPHY CORE FACILITY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 约书亚萨孔，X射线核心设施的主任，已经在COBRE中心建立了一个非常全面的结构生物学项目。 他解决了胶原蛋白结合域的X射线晶体结构，这是开发用于治疗骨质疏松症的甲状旁腺素-胶原蛋白结合域（PTH-CBD）融合蛋白的基础。 X射线核心设备包括配备Saturn 99 CCD探测器的Rigagku Cu阳极发生器、配备R-AXIS IV探测器的Rigaku Cr阳极发生器、Robotic Protein结晶仪器和计算机集群图形系统。 在1，298，000美元的总成本中，COBRE提供了390，000美元，其余部分由国防部陆军，能源部，NREL，NIH-BRIN和ABI的赠款资助。 该大学已承诺为永久员工电子专家杰里霍姆斯利和K.Z.辛来维护这些仪器。 虽然许多蛋白质晶体结构将使用新的X射线衍射仪器在内部完全解决，但更大，更困难的蛋白质结构将需要在国家同步加速器设施中收集衍射数据。 约书亚·萨肯（Joshua Sakon）正在广泛使用这些国家同步加速器光束线，并且是阿贡国家实验室先进光子源的注册用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Joshua Sakon, the director of the X-ray Core facility, has established a very comprehensive program in structural biology in the COBRE Center. He solved the X-ray crystal structure of the collagen binding domain which served as the basis for development of the parathyroid hormone-collagen binding domain (PTH-CBD) fusion protein for treatment of osteoporosis. The X-ray core facility includes a Rigagku Cu anode generator with a Saturn 99 CCD detector, a Rigaku Cr anode generator with R-AXIS IV detector, a Robotic Protein crystallization instrument, and computer cluster graphics systems. Of the total cost of $1,298,000, COBRE provided $390,000 while the remainder was funded with grants from DOD-Army, DOE, NREL, NIH-BRIN, and ABI. The University has committed institutional funds for permanent staff electronics specialists Jerry Homesley and K.Z. Shein to maintain these instruments. While many of the protein crystal structures will be completely solved in house using the new X-ray diffraction instrumentation, the larger, more difficult protein structures will require collection of diffraction data at national synchrotron facilities. Joshua Sakon is making extensive use of these national synchrotron beam lines, and is a registered user of the Advanced Photon Source at the Argonne National Laboratory.