MAINTENANCE AND REPAIR OF THE IVEM F30

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在本报告所述期间(6/1/08-5/31/09)，F30的维修工作最少(68小时)。我们获得了 共有875个倾斜系列(165个低温和633个常温)。这台显微镜用得很多 在典型的工作日(8-6M-F)，我们89%的工作都是在这些时间完成的。显微镜 在去年260个可用工作日中，有221个工作日被使用。此外，这台显微镜大约用了30分钟 每周工作时间(1553小时/52周)。F30用于室温断层扫描的比例为56%，22% 冷冻断层扫描，12%冷冻成像，4%室温成像，4%用于服务，2%用于 发展，培训为0%。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. For this reporting period (6/1/08-5/31/09), the F30 had minimal service work (68 hours). We acquired a total of 875 tilt-series (165 cryo and 633 room temperature). The microscope was heavily used during the typical workday (8-6 M-F) with 89% of our work done during those hours. The microscope was used 221 of the 260 available workdays last year. In addition, the microscope was used about 30 hours/week (1553 hours/52 weeks). The F30 was used 56% for room temperature tomography, 22% for cryo-tomography, 12% cryo-imaging, 4% for room temperature imaging, 4% for service, 2% for development, and 0% for training.