DESIGN/CONSTRUCT/TEST A NEW MASS SPECTROM FOR PROFILING PHOSPHORYLATION SITES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们设计并构建了一种新型的质谱仪，用于分析蛋白质上的磷酸化位点。该仪器的灵敏度比目前市售的仪器高出三个数量级。我们已经测试了原型，发现它按照设计和我们的模拟工作。我们已经为我们的新设计申请并获得了美国专利。 已经发表了一篇描述这项工作的论文： S. Myung，H. Cohen，D. J.C.费尼Padovan，A.N. Krutchinsky，B.T.查特“高容量离子阱耦合到飞行时间质谱仪，用于无扫描损失的综合链接扫描”，国际质谱杂志2010年。In press

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have designed and constructed a novel mass spectrometer for profiling phosphorylation sites on proteins. The instrument is designed to be up to three orders of magnitude more sensitive than current commercially available instrumentation. We have tested the prototype and found that it works as per its design and our simulations. We have applied for and being granted a US patent for our novel design. A paper describing this work has been published: S. Myung, H. Cohen, D. Feny¿, J.C. Padovan, A.N. Krutchinsky, B.T. Chait. "High-capacity ion trap coupled to a time-of-flight mass spectrometer for comprehensive linked scans with no scanning losses", International Journal of Mass Spectrometry 2010. In press