A NOVEL HIGH-CAPACITY ION TRAP-QUADRUPOLE TANDEM MASS SPECTROMETER

新型大容量离子阱四极杆串联质谱仪

• 批准号: 8361486
• 负责人: SUNNIE MYUNG
• 金额: $ 2.61万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361486
• 关键词: Coupled; Development; Funding; Generations; Grant; International; Ions; Journals; Legal patent; Link; Mass Spectrum Analysis; National Center for Research Resources; Paper; Peptides; Performance; Phosphopeptides; Principal Investigator; Publishing; Research; Research Infrastructure; Resources; Scanning; Source; TANDEM; Time; United States National Institutes of Health; Work; cost; instrument; macromolecule; mass spectrometer; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have recently developed a novel high capacity ion trap-quadrupole mass spectrometer, which we have described previously: A.N. Krutchinsky, H. Cohen, B.T. Chait A novel high-capacity ion trap-quadrupole tandem mass spectrometer Int J Mass Spectrom, 268 (2007) 93-105 and for which we have been granted a US patent. We have continued this development by constructing a second generation instrument as described below: A high-capacity ion trap coupled to a time-of-flight(TOF) mass spectrometer has been developed to carry out comprehensive linked scan analysis of all stored ions in the ion trap. The approach involves a novel tapered geometry high-capacity ion trap that can store more than 10^6 ions (range 8004000 m/z) without degrading its performance. Ions are stored and scanned out from the high-capacity ion trap as a function of m/z, collisionally fragmented and analyzed by TOF. Accurate mass analysis is achieved on both the precursor and fragments of all species ejected from the ion trap. We demonstrate the approach for comprehensive linked-scan identification of phosphopeptides in mixtures with their corresponding unphosphorylated peptides. A paper describing this work has been published: S. Myung, H. Cohen, D. Feny¿, J.C. Padovan, A.N. Krutchinsky, B.T. Chait. "High-capacity ion trap coupled to a time-of-flight mass spectrometer for comprehensive linked scans with no scanning losses", International Journal of Mass Spectrometry 2010. In press

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们最近开发了一种新型的高容量离子陷阱-四极质谱计，我们之前已经描述过： Krutchinsky，H.Cohen，B.T.Chait一种新型的高容量离子陷阱-四极串联质谱仪Int J质谱计，268(2007)93-105 我们已经为此获得了一项美国专利。 我们继续这一发展，建造了第二代仪器，如下所述： 研制了一种与飞行时间质谱仪(TOF)相耦合的大容量离子陷阱，用于对离子陷阱中存储的所有离子进行全面的连锁扫描分析。该方法包括一种新的锥形几何结构的高容量离子陷阱，它可以存储超过106个离子(范围8004000 m/z)，而不会降低其性能。作为m/z的函数，离子被存储并从高容量离子陷阱扫描出来，被碰撞碎裂，并通过TOF进行分析。对离子陷阱射出的所有物种的前体和碎片都进行了准确的质量分析。我们展示了一种对混合物中的磷酸肽及其相应的非磷酸化多肽进行全面联动扫描鉴定的方法。一篇描述这项工作的论文已经发表： 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容《高容量离子陷阱与飞行时间质谱仪相结合，实现无扫描损失的全面链接扫描》，《国际质谱学杂志》，2010年。在媒体上