INTERACTORS OF GDOWN1

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的合作者先前已经从组织提取物中分离出一种独特而丰富的哺乳动物Pol II亚群，该亚群含有一种额外的紧密相关的多肽Gdown 1。他们的研究结果表明，含有Gdown 1的Pol II（称为Pol II（G））选择性依赖于并响应于Mediator。我们目前正在对与Gdown 1相互作用的蛋白质进行彻底的质谱分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our collaborators have previously isolated from tissue extracts a distinct and abundant mammalian Pol II subpopulation that contains an additional tightly associated polypeptide, Gdown1. Their results established that Gdown1-containing Pol II, designated Pol II(G), is selectively dependent on and responsive to Mediator. We are currently carrying out a thorough mass spectrometric analysis of proteins that interact with Gdown1