Genomics Core Facility

基因组学核心设施是一个高质量的微阵列处理站点，任何NIDDK P.I.都可以访问该站点进行基因表达分析。如果时间允许，我们的服务也适用于其他NIH IC。核心工作人员从调查人员那里获取各种样本RNA，检查样本质量，制作探针，杂交和清洗表达阵列，收集数据，并将所有数据存储在DDK工作人员可以访问的中央数据库中。我们的生物信息师还为所有客户提供基本的数据分析，以确保数据质量。如可行，可按要求提供额外的分析。为了提高效率和重复性，我们采用了单一阵列平台。 在过去的一年里，我们扩大了我们的高容量并行测序服务。使用Illumina平台，我们从研究人员那里获得选定大小的样本，生成流动细胞簇，并捕获图像/序列数据。这些数据然后被转换成短标签(26-75nt)序列信息的文本文件，并传回给个别调查人员进行分析。目前，我们为这些数据提供了有限的生物信息学支持，分析的大部分负担都落在了个体研究人员的身上。这项服务对所有DDK私家侦探开放。

The Genomics Core Facility is a high quality microarray processing site that can be accessed by any NIDDK P.I. for gene expression analysis. Time permitting, our services are available to other NIH ICs. The Core Staff acquires various sample RNAs from investigators, checks sample quality, makes probes, hybridizes and washes expression arrays, collects data, and deposits all data in a central database that is accessible to DDK staff. Our bioinformatician also provides basic data analysis to all customers to ensure data quality. Additional analysis is provided as requested when feasible. For efficiency and reproducibility we have adopted a single array platform. In the past year, we have expanded our high capacity, parallel sequencing services. Using the Illumina platform, we acquire size-selected samples from researchers, generate flow cell clusters, and capture image/sequence data. That data is then converted to text files of short tag (26-75nt) sequence information and passed back to individual investigators for analysis. We provide limited bioinformatic support for this data at this time with the bulk of the burden for analysis being placed on the individual investigators. This service is open to all DDK P.I.s.