Core D: Advanced Imaging

项目总结（见说明）： 哥伦比亚大学医学中心皮肤病研究中心的高级成像核心将提供最先进的显微镜的指导和专业培训，用于整个动物，体内，活细胞和皮肤及其细胞的高内容成像。随着多功能基因编码荧光报告基因的出现以及能够对厚标本和活组织进行成像的多光子显微镜的日益普及，三维、活细胞和延时成像现在已成为现代生物医学武器库中的重要工具。此外，皮肤对成像研究的特殊可及性提供了 这是一个前所未有的机会，可以识别在广泛的时间（毫秒到数月）和空间（亚细胞到整个动物）尺度上连接皮肤不同细胞类型的功能网络。 核心的长期目标是使CSDRC研究人员能够使用先进的成像技术来提高他们以皮肤为重点的研究的有效性，并完成传统显微镜，生物化学或组织学无法完成的研究。核心的创新特点是：1）致力于为中心研究人员、博士后研究员和研究生提供一对一的专业培训，使他们能够掌握在研究中成功使用先进显微镜所需的技能; 2）其工作人员是在体内使用方面具有丰富经验的成像专家。 以及皮肤及其细胞的活细胞成像。核心的服务将包括多光子显微镜，体内小动物成像，共聚焦显微镜，活细胞成像和高内涵成像。 通过使整个动物在体内和活细胞成像方法广泛提供给皮肤生物学家，核心旨在彻底改变皮肤病学研究，从传统的固定组织的组织学研究转向动态分析的活皮肤。具体目标是： 1.通过体内成像对完整皮肤中的细胞类型进行功能分析。 2.通过提供高内涵的成像服务，在体外有效分析细胞机制。 3.实现皮肤中分子和细胞的高分辨率三维结构成像。

PROJECT SUMMARY (See instructions): The Advanced Imaging Core of the Columbia University Medical Center Skin Diseases Research Center will provide guidance and specialized training on state-of-the-art microscopes for whole-animal, in vivo, live-cell and high-content imaging of the skin and its cells. With the advent of versatile genetically encoded fluorescent reporters and the increasing availability of multiphoton microscopes capable of imaging thick specimens and living tissues, three-dimensional, live-cell and time-lapse imaging are now essential tools in the modem biomedical arsenal. Moreover, the skin's exceptional accessibility for imaging studies provides an unprecedented opportunity to identify functional networks that link the skin's diverse cell types over broad temporal (milliseconds to months) and spatial (subcellular to whole animals) scales. The Core's long-term objective is to enable CUMCSDRC investigators to use sophisticated imaging techologies to increase the effectiveness of their skin-focused research and to accomplish studies that are not possible with traditional microscopy, biochemistry or histology. The Core's innovative features are 1) its commitment to one-on-one specialized training for Center investigators, postdoctoral fellows and graduate students, so that they develop the skills necessary to successfully employ sophisticated microscopes in their research, and 2) its personnel, who are imaging experts who have extensive experience in using in vivo and live-cell imaging of the skin and its cells. The Core's services will include multiphoton microscopy, in vivo small-animal imaging, confocal microscopy, live-cell imaging and high-content imaging. By making whole-animal in vivo and live-cell imaging approaches widely available to skin biologists, the Core seeks to revolutionize dermatologic research by shifting away from traditional histological studies of fixed tissue toward dynamic analysis of the living skin. The Specific Aims are: 1. To enable functional analysis of cell types in the intact skin with in vivo imaging. 2. To efficiently analyze cellular mechanisms in vitro by providing high-content imaging services. 3. To enable high-resolution, three-dimensional structural imaging of molecules and cells in the skin.