Core A Verkman Screening Core

项目总结(见说明)： 高通量筛选核心将继续根据定义的靶标或表型对小分子文库进行筛选。该中心目前的资源包括超过250,000个类药物化合物和较小的纯化天然化合物和批准/研究药物的集合，以及利用光学(荧光，吸光度， 发光)检测。核心的功能包括化合物存储和处理、化验设计和验证、化验执行和数据分析。通过对商业购买的化合物类似物进行筛选，初步优化了有效的‘HITS’(具有针对特定靶点或表型的活性的化合物)。 在与核心部门协商的情况下，在个别项目中进行进一步的“命中到领先”开发。该核心在本项目期间得到了广泛利用，成功地确定和表征了离子转运体和感染的新型调节剂。这一核心将被28个正在进行的和新的与气候变化有关的项目大量使用。

PROJECT SUMMARY (See instructions): The high-throughput screening core will continue to carry out screening of small-molecule libraries against defined targets or phenotypes. Resources of the core at present include more than 250,000 drug-like compounds and smaller collections of purified natural compounds and approved/investigational drugs, and automated instrumentation for high-throughput screening utilizing optical (fluorescence, absorbance, luminescence) assays. Functions of the core include compound storage and handling, assay design and validation, assay execution, and data analysis. Initial optimization of validated 'hits' (compounds with activity against defined targets or phenotypes) is done by screening of commercially purchased compound analogs. Further 'hit-to-lead' development is done in individual projects in consultation with the core. The core has been utilized extensively during the current project with successful identification and characterization of novel modulators of ion transporters and infection. The core will be heavily utilized by 28 ongoing and new CF related projects.