Core C finkbeiner Cell Models Core

项目摘要(请参阅说明)：细胞模型核心的目标是提供细胞培养模型，用于小分子CF药物的发现、先导化合物的测试、与治疗相关的临床前生物测定，以及CF和呼吸道病理生理学的翻译科学研究。这些目标是通过为研究人员提供人类CF和非CF呼吸道表面上皮和粘膜腺体细胞的高度分化细胞培养模型；呼吸道上皮细胞和腺细胞的新型球形培养；以及与CF药物发现相关的癌和转化细胞系的培养来实现的。核心资源包括冷冻保存的CF和非CF呼吸道上皮细胞，各种肿瘤和永生化细胞系，以及用于细胞分离、细胞培养和细胞模型质量测试的仪器。核心的具体功能包括建立呼吸道表面上皮细胞和粘膜下腺细胞的高分化细胞模型；细胞的冷冻保存，以确保研究人员的持续供应；扩展有价值的细胞，以增加研究人员的资源；培养对CF药物开发和翻译科学研究有用的细胞系；对原代细胞培养和细胞系进行质量控制测试；以及最后，向参与研究的科学家提供相关的细胞模型。我们的核心实验室已经为CF研究人员免费提供了超过25年的细胞。本次续订中描述的20多个项目将使用提供的单元模型。核心继续开发和研究新的细胞模型，以确保提供最好的和最先进的模型。除了我们在提供呼吸道表面上皮和粘膜下腺培养方面的长期实力外，CORE(与临床材料核心合作)已经积累了广泛的经验，可以从不同基因类型的CF患者和非CF对照组获得鼻黏膜上皮平面和椭圆形培养

PROJECT SUMMARY (See instructions): The objectives of the Cell Models Core are to provide cell culture models for use in small-molecule CF drug discovery, testing of lead compounds, therapeutically relevant preclinical bioassays, and translational science investigations of CF and airway pathophysiology. The objectives are accomplished by providing investigators highly differentiated cell culture models of human CF and non-CF respiratory tract surface epithelial and sumucosal gland cells; novel spheroid cultures of the respiratory tract epithelial and gland cells; and cultures of carcinoma and transformed cell lines relevant for CF drug discovery. Resources of the core include cryopreserved CF and non-CF respiratory tract epithelial cells, various neoplastic and immortalized cell lines, and instrumentation for cell isolation, cell culture and quality testing of the cell models. Specific functions of the core include the generation of highly differentiated cell models of respiratory tract surface epithelial cells and submucosal gland cells; cryopreservation of cells to insure continuous supplies for investigators; expanding valuable cells to increase resources for investigators; culturing cell lines useful in CF drug discovery and translational science studies; quality control testing of primary cell cultures and cell lines; and finally, supplying relevant cell models to participating scientists. Our core laboratory has provided cells to CF investigators for over twenty-five years free of charge. More than 20 projects described in this renewal will use the cell models provided. The core continues to develop and investigate new cell models to ensure that the best and state-of-the-art models are provided. In addition to our long-established strength in providing airway surface epithelial and submucosal gland cultures, the core (in collaboration with the Clinical Materials Core) has developed extensive experience generating nasal epithelial planar and spheroid cultures obtained from CF patients of various genotypes and non-CF controls