Core C finkbeiner Cell Models Core

项目概述（见说明）：细胞模型核心的目标是提供细胞培养模型，用于小分子CF药物发现、先导化合物测试、治疗相关的临床前生物测定以及CF和气道病理生理学的转化科学研究。研究人员通过提供人类CF和非CF呼吸道表面上皮细胞和粘膜细胞的高度分化细胞培养模型来实现这一目标；呼吸道上皮细胞和腺体细胞的新型球形培养；以及与CF药物发现相关的癌细胞和转化细胞系的培养。核心资源包括冷冻保存的CF和非CF呼吸道上皮细胞，各种肿瘤和永生化细胞系，以及用于细胞分离，细胞培养和细胞模型质量测试的仪器。核心的具体功能包括产生呼吸道表面上皮细胞和粘膜下腺细胞的高分化细胞模型；细胞的低温保存，以确保调查员的持续供应；扩大有价值的小组，增加调查人员的资源；培养可用于CF药物发现和转化科学研究的细胞系；原代细胞培养和细胞系的质量控制试验最后，为参与的科学家提供相关的细胞模型。我们的核心实验室为CF研究人员免费提供细胞超过25年。本更新中描述的20多个项目将使用所提供的单元模型。核心继续开发和研究新的细胞模型，以确保提供最好的和最先进的模型。除了我们长期以来在提供气道表面上皮和粘膜下腺培养方面的优势外，核心（与临床材料核心合作）已经积累了丰富的经验，从不同基因型的CF患者和非CF对照中获得鼻腔上皮平面和球形培养

PROJECT SUMMARY (See instructions): The objectives of the Cell Models Core are to provide cell culture models for use in small-molecule CF drug discovery, testing of lead compounds, therapeutically relevant preclinical bioassays, and translational science investigations of CF and airway pathophysiology. The objectives are accomplished by providing investigators highly differentiated cell culture models of human CF and non-CF respiratory tract surface epithelial and sumucosal gland cells; novel spheroid cultures of the respiratory tract epithelial and gland cells; and cultures of carcinoma and transformed cell lines relevant for CF drug discovery. Resources of the core include cryopreserved CF and non-CF respiratory tract epithelial cells, various neoplastic and immortalized cell lines, and instrumentation for cell isolation, cell culture and quality testing of the cell models. Specific functions of the core include the generation of highly differentiated cell models of respiratory tract surface epithelial cells and submucosal gland cells; cryopreservation of cells to insure continuous supplies for investigators; expanding valuable cells to increase resources for investigators; culturing cell lines useful in CF drug discovery and translational science studies; quality control testing of primary cell cultures and cell lines; and finally, supplying relevant cell models to participating scientists. Our core laboratory has provided cells to CF investigators for over twenty-five years free of charge. More than 20 projects described in this renewal will use the cell models provided. The core continues to develop and investigate new cell models to ensure that the best and state-of-the-art models are provided. In addition to our long-established strength in providing airway surface epithelial and submucosal gland cultures, the core (in collaboration with the Clinical Materials Core) has developed extensive experience generating nasal epithelial planar and spheroid cultures obtained from CF patients of various genotypes and non-CF controls