Q-Exactive Orbitrap LC-MC/MS System

描述（由申请人提供）：本提案要求为Q-Exactive Orbitrap LC-MS/MS系统提供资金，该系统将放置在特拉华州大学化学和生物化学系的质谱设施中。为了最大限度地发挥这一“主力”仪器的效用，它将配置电喷雾电离（ESI）和大气压化学电离（APCI）源，以及具有快速柱切换能力的常规流动LC和用于肽分析的纳米LC。该仪器将提供特拉华州大学（UD）目前缺乏的研究能力。目前，UD的研究人员无法在现场或州内使用高分辨率精确质谱（HRMS）仪器来表征小分子。该仪器将为这些研究人员提供这一关键工具。此外，Orbitrap LC-MS/MS将使蛋白质和肽样品的MS分析成为UD许多生物医学研究项目的关键。Orbitrap LC-MS/MS的高灵敏度和广泛的覆盖范围将优于UD研究人员持续使用的老化Q-ToF仪器的上级。明确的管理计划和高级别的机构承诺确保了这一广泛使用的工具的可持续性。拟议的仪器将为NIH资助的6个主要和7个次要用户的研究计划提供关键支持，这些用户涵盖生物化学，化学和材料科学。LC-MS/MS将极大地促进广泛的生物医学研究领域的进步，包括结构酶学，合成方法开发，蛋白质组装，酶的信号传导和调控途径，分子设计，组织再生和诊断设备的材料科学以及化学探针发现。该仪器还将为特拉华州大学的几个主要NIH计划提供支持，包括化学生物学界面（CBI）培训计划，特拉华州校园内的多个生物医学研究卓越中心（COBRE），特拉华州IDEA生物医学研究卓越网络（INBRE）和转化癌症研究中心（CTCR）。

DESCRIPTION (provided by applicant): This proposal requests funding for a Q-Exactive Orbitrap LC-MS/MS system to be placed in the Mass Spectrometry Facility of the Department of Chemistry and Biochemistry at the University of Delaware. To maximize the utility of this "workhorse" instrument, it will be configured with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources, as well as a conventional-flow LC with fast column- switching capabilities and a nano-LC for analysis of peptides. This instrument will provide research capabilities that are currently lacking at the University of Delaware (UD). Currently, researchers at UD have no on-site or in-state access to high-resolution accurate mass mass spectrometry (HRMS) instruments for characterization of small molecules. This instrument will provide this crucial tool to these researchers. Further, the Orbitrap LC- MS/MS will enable MS analysis of protein and peptide samples essential for many biomedical research programs at UD. The high sensitivity and broad coverage of the Orbitrap LC-MS/MS will be superior to that of the aging Q-ToF instrument, which is in continuous use by UD researchers. A well-defined management plan and high level of institutional commitment ensure the sustainability of this broadly used instrument. The proposed instrument will provide critical support for NIH-funded research programs of 6 major and 7 minor users spanning biochemistry, chemistry and materials science. The LC-MS/MS will greatly enable advances across a wide range of biomedical research areas, including structural enzymology, synthetic method development, protein assemblies, signaling and regulatory pathways for enzymes, molecular design, materials science for tissue regeneration and diagnostic devices, and chemical probe discovery. The instrument will also provide support to several major NIH initiatives at the University of Delaware, including the Chemistry-Biology Interface (CBI) Training Program, multiple Centers of Biomedical Research Excellence (COBRE) on Delaware's campus, the Delaware IDeA Network of Biomedical Research Excellence (INBRE), and the Center for Translational Cancer Research (CTCR).