LC-Orbitrap MS/MS System for shotgun Proteomics at Dartmouth

描述（由申请人提供）：本提案的目的是为微毛细管LC-LTQ Orbitrap Elite质谱仪系统获得资金，以支持更大达特茅斯生物医学研究社区的以鸟枪蛋白质组学为导向的NIH资助的科学目标。在本申请中描述的所提出的系统的用户来自达特茅斯学院和达特茅斯医学院的广泛的部门和中心，包括NCI指定的诺里斯棉花癌症中心（NCCC）。他们的项目代表了一个多样化的研究焦点，从细胞周期控制和昼夜节律的基本细胞机制到宿主-病原体相互作用和胰岛素信号传导的机制，再到旨在了解细胞信号传导如何在人类肺癌中失调的更多翻译项目。总的来说，这些研究项目代表的工作和努力已经超过了达特茅斯所有这些实验中存在的单一LC-MS/MS平台的能力。因此，收购一个新的质谱仪到达特茅斯蛋白质组学共享资源是至关重要的，使当前的完成，以及未来，我们的教师NIH的研究目标。拟议的仪器将被安置在我们的蛋白质组学共享资源，这是由NCCC代表更大的达特茅斯社区维护。

DESCRIPTION (provided by applicant): The objective of this proposal is to secure funding for a microcapillary LC-LTQ Orbitrap Elite mass spectrometer system to support shotgun proteomics-oriented, NIH-funded scientific goals of the greater Dartmouth biomedical research community. The users of the proposed system described in this application come from a broad range of departments and centers at Dartmouth College and Dartmouth Medical School, including the NCI-designated Norris Cotton Cancer Center (NCCC). Their projects represent a diverse continuum of research foci, from basic cellular mechanisms of cell cycle control and circadian rhythms to mechanisms of host-pathogen interactions and insulin signaling, to more translational projects aimed at understanding how cellular signaling is dysregulated in human lung cancers. Collectively, these research projects represent work and effort that has exceeded the capacity of the single LC-MS/MS platform that exists in all of Dartmouth for such experiments. Thus, the acquisition of a new mass spectrometer into the Dartmouth Proteomics Shared Resource is critically important to enable the completion of current, as well as future, NIH research goals of our faculty. The proposed instrumentation will be housed in our Proteomics Shared Resource, which is maintained by NCCC on behalf of the greater Dartmouth community.