PROTEOMICS SHARED RESOURCE

蛋白质组学共享资源

• 批准号: 7944667
• 负责人: Scott A. Gerber
• 金额: $ 6.67万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-21 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944667
• 关键词: Acetylation; Address; Administrator; Alkylation; Amino Acid Sequence; Archives; Area; Arts; Back; Bar Codes; Bioinformatics Shared Resource; Biology; Bite; Businesses; Cancer Center; Cancer Center Support Grant; Charge; Client; Code; Commit; Communities; Complex Mixtures; Computer Assisted; Computer software; Computers; Consult; Consultations; Core Facility; Cost Analysis; Cost Savings; DNA; Data; Data Analyses; Data Files; Data Reporting; Databases; Digestion; Drops; Electronic Mail; Electronics; Electrophoresis; Ensure; Equipment; Experimental Designs; Faculty; Feedback; Foundations; Funding; Future; Gel; Grant; Growth; High Pressure Liquid Chromatography; Hour; Housing; Immunoprecipitation; Individual; Institution; Ions; Laboratories; Laboratory Research; Maintenance; Manuals; Mass Spectrum Analysis; Methods; Methylation; Mission; Modification; Molecular Biology; Molecular Weight; Names; Norris Cotton Cancer Center; Operative Surgical Procedures; Peptide Sequence Determination; Peptides; Phase Transition; Phosphorylation; Post Translational Modification Analysis; Post-Translational Protein Processing; Price; Process; Protein Analysis; Proteins; Proteolysis; Proteomics; Proteomics Shared Resource; Protocols documentation; Pump; Recruitment Activity; Reporting; Research; Research Personnel; Resource Sharing; Resources; Running; Sampling; Security; Services; Silver; Silver Staining; Site; Solvents; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staging; Staging System; Staining method; Stains; Structure; System; Technology; Time; Training; Trypsin; Update; Validation; Writing; base; chemical synthesis; cluster computing; computing resources; cost; data acquisition; data integrity; design; detector; encryption; falls; firewall; functional group; instrument; instrumentation; interest; liquid chromatography mass spectrometry; mass spectrometer; medical schools; meetings; member; parallel processing; protein complex; research study; skills; small molecule; trafficking; web site

The mission of the Proteomics Shared Resource (PRSR) at the Norris Cotton Cancer Center (NCCC) is to provide NCCC investigators with local access to state-of-the-art proteomic technologies, scientific consulting For experimental design, consultation regarding results interpretation, and ancillary bioanalytical support services at affordable rates. Newly established as an independent NCCC Shared Resource, the PRSR in Fall 2007 completed its transition from providing limited proteomic services as part of the former Molecular Biology and Proteomics Shared Resource to full-fledged independent operation as a stand-alone core facility. During this transition, the PRSR acquired newly renovated laboratory space in the NCCC Rubin Building research labs, modernized its mass spectrometry and liquid chromatography instrumentation and expanded its computational resources, appointed proteomics faculty recruit Dr. Scott A. Gerber as Director of the PRSR, and expanded its service portfolio to include protein post-translational modification analysis and complex protein mixture analysis. These efforts collectively represent a $2.8 million commitment from the NCCC and From Dartmouth Medical School to bring a modern proteomics platform within reach of Cancer Center investigators and the greater Dartmouth research community as a whole. During this one-year transition phase, the PRSR received $12,700 in chargebacks from 9 NCCC research laboratories, or 87% of the total ($14,600) chargebacks for the facility (from a collective 16 Dartmouth laboratories). We estimate that the total operating cost of the PRSR for FY 2008 (year 1 of the CCSG renewal) will be $326,760 and anticipate $40,000 in total chargebacks, requiring $48,349 from CCSG and $238,411 from other sources for NCCC investigators (75% of the shortfall). Owing to the rapid increase in demand for PRSR services over the past year, we anticipate strong growth in the rate of chargebacks for the PRSR such that the large subsidy by NCCC and the institution can be reduced. Currently, support funds for the PRSR in transition are provided by multiple institutional sources.

诺里斯棉花癌症中心（NCCC）的蛋白质组学共享资源（PRSR）的使命是 为NCCC研究人员提供当地最先进的蛋白质组学技术，科学咨询， 用于实验设计、结果解读咨询和辅助生物分析支持 以负担得起的价格提供服务。 作为一个独立的NCCC共享资源，PRSR于2007年秋季完成了其 从提供有限的蛋白质组学服务，作为前分子生物学和蛋白质组学的一部分， 共享资源作为一个独立的核心设施全面独立运作。在这一过渡期间， PRSR在NCCC鲁宾大楼研究实验室获得了新装修的实验室空间， 现代化了质谱和液相色谱仪器，并扩大了 计算资源，任命蛋白质组学教师招募博士斯科特A。戈贝尔作为PRSR的主任， 并扩大了其服务组合，包括蛋白质翻译后修饰分析和复杂的 蛋白质混合物分析这些努力总共代表了NCCC承诺的280万美元， 来自达特茅斯医学院，为癌症中心带来现代蛋白质组学平台 调查人员和更大的达特茅斯研究界作为一个整体。 在为期一年的过渡阶段，PRSR从9个NCCC研究中获得了12，700美元的退款 实验室，或87%的总（14,600美元）拒付的设施（从集体16达特茅斯 实验室）。我们估计，2008财年（CCSG第1年）PRSR的总运营成本 续订）将为326，760美元，预计总退款为40，000美元，需要CCSG提供48，349美元， 238，411美元来自其他来源，用于NCCC调查员（短缺的75%）。由于快速增长， 过去一年对PRSR服务的需求，我们预计 PRSR使得NCCC和机构的大量补贴可以减少。目前，支持资金 过渡中PRSR由多个机构来源提供。