Zeiss 780 NLO High Sensitivity Multiphoton Microscopy System

描述（由申请人提供）：wm Keck细胞成像中心（KCCI）是国际公认的先进光学显微镜成像中心，以蛋白质-蛋白质相互作用成像而闻名，现申请将现有的11年历史的Biorad Radiance2100共聚焦/多光子显微镜系统替换为带有QUASAR 34通道GaAasP高灵敏度探测器和非扫描（GaAasP）探测器（NDD）的蔡司780 NLO。我们还要求用数字可调谐变色龙Vision-S超快激光系统取代已有11年历史的手动可调谐相干Mira 900系统，该系统将与蔡司780 NLO系统耦合。KCCI是一个大学范围内的成像设施，位于所有参与研究人员的步行距离内。所要求的仪器将使这所大学的基础和临床科学家受益；特别是10名参与联邦资助的调查人员（主要使用者）和5名次要使用者。所要求的蔡司780 NLO系统具有相干变色龙视觉- s 2p激光器是独一无二的，并且为用户提供了数字调谐的能力，以选择激发波长（通过软件），以准确匹配实验样品中的荧光团。高量子效率（在540nm处为45%）的GaAsP探测器提供了更好的机会，以较低的激发强度激发样品，并检测组织和活体成像中的较弱光子。该仪器对于我们的核心研究小组来说是必不可少的，并且对于培训其他对组织和活体动物中各种生物应用研究感兴趣的研究人员和参加秋季学期显微镜课程的学生也是至关重要的。目前，弗吉尼亚大学还没有蔡司780 NLO系统，该系统具有用于活体或深部组织成像的690至1050 nm的数字可调激光多光子显微镜。KCCI因其在弗吉尼亚大学举办了11年的年度实践培训工作坊而获得认可。所要求的蔡司780 NLO是一种先进的技术，将帮助我们推进分子成像的方法，这将帮助我们继续我们的推广计划，如我们的年度FRET研讨会。总之，所要求的仪器将为弗吉尼亚大学及其他研究界提供目前无法获得的成像能力水平，这将大大加强正在进行和未来的研究。

DESCRIPTION (provided by applicant): The W.M. Keck Center for Cellular Imaging (KCCI), an internationally recognized center for advanced light microscopy imaging that is particularly known for protein-protein interaction imaging, is requesting to replace the existing 11 years old Biorad Radiance2100 confocal/multiphoton microscopy system with a Zeiss 780 NLO with a QUASAR 34-channel GaAasP high-sensitivity detector and non-descan (GaAasP) detector (NDD). We are also requesting to replace the 11 years old manually tunable Coherent Mira 900 system with a digitally-tunable Chameleon Vision-S ultrafast laser system, to be coupled to the Zeiss 780 NLO systems. KCCI is a University-wide imaging facility, located within walking distance of all the participating investigators. The requested instrument will benefit the basic an clinical scientists of this university; in particular the 10 participating federally funded investigators (Major users) and 5 minor users. The requested Zeiss 780 NLO system with Coherent Chameleon Vision-S 2p laser is unique and, providing users the ability to digitally tune to select the excitation wavelengths (through software) to match exactly the fluorophores in their experimental samples. The higher quantum efficiency (45% at 540nm) GaAsP detector provides better opportunity to excite the sample with less excitation intensity and detecting the weaker photons in tissue and intravital imaging. This instrument is essential for our core investigator group, and will also be critical for training other investigators and students attending the fall semester microscopy course interested in the study of various biological applications in tissues and live animals. There is currently no Zeiss 780 NLO system available at the University of Virginia that has a digitally tunable laser from 690 to 1050 nm for multiphoton microscopy for intravital or deep tissue imaging. KCCI is recognized for its annual hands- on training FRET workshop, held for the past eleven years at the University of Virginia. The requested Zeiss 780 NLO is an advanced technology would help us to advance the methodology in molecular imaging and that will help us to continue our outreach program such as our annual FRET workshop. In summary, the requested instrument will provide the research community at the University of Virginia and beyond with a level of imaging capability that is not currently availabl, and this will enhance significantly ongoing and future research.