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Multiphoton Spectral Imaging Microscopy

多光子光谱成像显微镜

基本信息

批准号：
6877502
负责人：
AMMASI PERIASAMY
金额：
$ 50万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2006-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877502
关键词：
biomedical equipment purchase cell biology confocal scanning microscopy fluorescence microscopy fluorescence resonance energy transfer molecular /cellular imaging protein protein interaction tissues

项目摘要

DESCRIPTION (provided by applicant): The W.M. Keck Center for Cellular Imaging (KCCI), a leading center in advanced light microscopy imaging, particularly in protein-protein interaction imaging, is requesting a Zeiss "Multiphoton Spectral Imaging Microscopy (MSIM)" system. KCCI is a University wide imaging facility and the requested instrument will benefit the basic and clinical science investigators of this university, and in particular the 8 participating Federally funded investigators. The instrument will be housed in the well-established KCCI, located within walking distance of all the participating investigators. The central theme of the research to be performed on the requested Zeiss MSIM is the study of protein-protein interactions in live cells and tissues including: liganddependent protein interactions between ERalpha and corepressor proteins; mitochondria! disruption in traumatic axonal pathogenesis; FRET Imaging of Rho family GTPases; association of tau protein in Alzheimer's disease; the pattern of strain fibronectin networks during gastrulation; the dynamics of astroglial processes and their interactions with injured neurons; gene regulatory mechanisms in transgenic embryos; and cellular uptake of anti-cancer drugs. This system allows the application of biophysical techniques such as fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET), to live specimens, in situ. Our request for the Zeiss MSIM is in response to the growing need of researchers at this institution to take advantage of these technological advances in their NIH-funded projects. KCCI is an internationally known center in the study of protein-protein interactions using various light microscopy techniques and provides hands on training annually for scientists from the United States as well as scientists from 15 countries. Currently, we do not have a multiphoton spectral imaging system, this is essential for our investigators, as well as for training others, to study the protein associations in living cells and tissues. In summary, the requested instrument will provide the research community at the University of Virginia with a level of imaging capability that has not been available in the past and will enhance ongoing and future research significantly.

描述(申请人提供)：W.M.凯克细胞成像中心(KCCI)是先进光学显微镜成像，特别是蛋白质-蛋白质相互作用成像领域的领先中心，该中心要求使用蔡司公司的“多光子光谱成像显微镜(MSIM)”系统。KCCI是一所大学范围内的成像设施，所要求的仪器将使该大学的基础和临床科学研究人员受益，特别是8名参与联邦资助的研究人员。该仪器将放置在久负盛名的KCCI内，所有参与调查的人员步行即可到达。要求进行的蔡司MSIM研究的中心主题是研究活细胞和组织中的蛋白质-蛋白质相互作用，包括：ERα和辅阻遏子蛋白之间的配体依赖性蛋白质相互作用；线粒体！这些研究包括：创伤轴突发病机制的改变；Rho家族GTP酶的FRET成像；tau蛋白与阿尔茨海默病的关系；原肠形成过程中菌株纤维连接蛋白网络的模式；星形胶质细胞突起的动态及其与受损神经元的相互作用；转基因胚胎中的基因调控机制；以及细胞对抗癌药物的吸收。该系统允许将荧光漂白后恢复(FRAP)和荧光共振能量转移(FRET)等生物物理技术应用于活标本的原位检测。我们对蔡司MSIM的请求是为了满足该机构研究人员在其NIH资助的项目中利用这些技术进步的日益增长的需求。KCCI是使用各种光学显微镜技术研究蛋白质-蛋白质相互作用的国际知名中心，每年为来自美国和15个国家的科学家提供动手培训。目前，我们没有多光子光谱成像系统，这对于我们的研究人员以及其他培训人员研究活细胞和组织中的蛋白质关联是必不可少的。总之，所要求的仪器将为弗吉尼亚大学的研究界提供过去没有的成像能力，并将显著加强正在进行的和未来的研究。