Molecular Basis of Oral Microbial Adhesion

产品说明：牙周病是由宿主和口腔微生物菌群之间的相互作用引起的支持牙齿的组织的复杂慢性炎症性病症。微生物在疾病的初始阶段起作用，作为激活宿主炎症反应的触发剂。这涉及宿主和细菌之间的一系列复杂的相互作用，然而迄今为止，介导这些相互作用的细菌和宿主组分仍然没有得到很好的理解。启动感染的一个重要步骤涉及病原体或其产物对宿主细胞的粘附。细菌细胞表面蛋白是最先与宿主环境接触的，并且最有可能是该过程的介质。虽然对需氧外膜和细胞表面蛋白质了解很多，但关于厌氧菌的知识却很少。厌氧菌，如牙龈卟啉单胞菌，坦纳氏菌和中间普氏菌与牙周病的发展有关。我们建议研究口腔厌氧菌的细胞表面蛋白在宿主中建立微生物的第一步：口腔定植中的作用。具体来说，首先我们将描述Prev。中间体17纤连蛋白结合蛋白，AdpA.其次，使用细胞标记和蛋白质组学方法的组合，我们将鉴定能够结合宿主组分的其他细胞表面蛋白：例如唾液蛋白、基底膜蛋白和宿主细胞。还将进一步表征这些蛋白质以确定它们在介导Prev附着中的作用。中间体到主机组件。本申请的长期目标是确定Prev。intermedia adhesins，这将进一步了解介质的细菌的发病机制，并最终导致潜在的抗牙周炎疫苗候选人的鉴定。

DESCRIPTION: Periodontal diseases are complex chronic inflammatory conditions of tissues supporting the teeth resulting from the interaction between host and oral microflora. Microorganisms play a role in the initial stages of the diseases serving as a trigger for activation of the host inflammatory response. This involves complex set of interactions between the host and the bacteria, however to date the bacterial and the host components mediating these interactions remain not well understood. An essential step in initiating the infection involves adherence to host cells by the pathogens or their products. Bacterial cell surface proteins are the first to come in contact with the host environment and are most likely mediators of this process. Although much is known about aerobic outer membrane and cell surface proteins this knowledge is sparse regarding anaerobic bacteria. Anaerobes such as Porphyromonas gingivalis, Tannerella forsythensis, and Prevotella intermedia are implicated in development of periodontal diseases. We propose to investigate the role of cell surface proteins of the oral anaerobic bacteria in the first step of establishment of the microorganisms in the host: colonization of the oral cavity. Specifically, first we will characterize the Prev. intermedia 17 fibronectin-binding protein, AdpA. Second, using combination of cell labeling and proteomic approaches we will identify other cell surface proteins capable of binding to host components: e.g. salivary proteins, basal membrane proteins, and host cells. These proteins also will be further characterized to determine their role in mediating the attachment of Prev. intermedia to host components. The long-term objective of this application is to identify Prev. intermedia adhesins, which will further knowledge of mediators of the pathogenesis of the bacterium and ultimately lead to identification of potential anti-periodontitis vaccine candidates.