Regulation and Control of Mineralization in Teeth

描述（由申请人提供）：拟议的研究旨在提高我们对如何使用仿生方法重新矿化龋牙釉质并适当恢复正常牙釉质结构和性能的理解。我们的工作假设是通过调节矿物离子扩散、晶体生长动力学和晶体取向来实现牙齿正常结构和功能的恢复或再生。研究将以一个有效的数学模型为指导，该数学模型将被开发出来，以描述这些关键事件的动力学和相互关系，该模型基于使用一种新的化学方法在体内样早期龋齿病变中再矿化产生的实验数据。以该模型为基础，研究牙结构再矿化和再生的仿生方法。提出的仿生策略是基于我们目前对矿物沉积和组织如何在发育矿化组织中受到调节的知识状态。鉴于龋齿的高发率，对比现有的修复方法更好的修复方法的需求是巨大的。这些研究的长期目标是开发新的方法来再生正常的牙齿结构和功能。总的来说，这些研究考虑了离子扩散和驱动人类牙釉质溶解和沉淀的重要性，以及调节牙釉质矿物晶体生长速度和形状的合成和生物相关分子的作用。具体而言，我们建议：1。探讨新型酸性再矿化液的体外作用机制及潜在效果；2. 确定唾液蛋白和肽稳定过饱和磷酸钙溶液的机制和再矿化效果；3.确定由焦磷酸盐稳定的新型过饱和磷酸钙溶液的再矿化效果，其中再矿化动力学由添加的磷酸酶调节；4.建立牙釉质裂隙病变再矿化的显微放射学、偏振光显微镜和x射线显微断层扫描系统；和5。确定稳定的无定形磷酸钙（ACP）作为成熟牙釉质晶体外延生长的前体的可能作用，特别关注龋齿牙釉质裂隙组织的再矿化和再生。

DESCRIPTION (provided by applicant): The proposed studies are designed to improve our understanding of how biomimetic approaches can be used to remineralize carious enamel and properly restore normal tooth enamel structure and properties. Our working hypothesis is that the restoration or regeneration of proper tooth structure and function can be achieved through the regulation of mineral ion diffusion, crystal growth kinetics and crystal orientation. Studies will be guided by a working mathematical model that will be developed to describe the dynamics and interrelationships of these key events, based on experimental data generated using a novel chemical approach to remineralize in vivo-like incipient carious lesions. Using this model as a basis, novel biomimetic approaches to remineralize and regenerate tooth structures will be studied. The proposed biomimetic strategies are based on our current state of knowledge of how mineral deposition and organization are regulated in developing mineralized tissues. Given the high prevalence of dental caries, there is a tremendous need for restorative procedures that are superior to those presently available. The long-term goal of these studies is to develop new procedures to regenerate normal tooth structure and function. In general, these studies consider the importance of ion diffusion and driving forces for dissolution and precipitation in human enamel, along with the role of synthetic and biologically relevant molecules that regulate the rate and shape of growing enamel mineral crystals. Specifically, we propose: 1. To determine the mechanism and potential effectiveness of novel acidic remineralizing solutions in vitro; 2. To determine the mechanism and remineralization effectiveness of supersaturated calcium phosphate solutions that are stabilized by selected salivary proteins and peptides; 3.To determine the remineralization effectiveness of novel supersaturated calcium phosphate solutions that are stabilized by pyrophosphate, where remineralization kinetics are regulated by added phosphatases; 4.To develop an in vitro system to study the remineralization of enamel fissure lesions using microradiography, polarized light microscopy and X-ray microtomography; and 5. To determine the possible role of stabilized amorphous calcium phosphate (ACP) as a precursor for the epitaxial growth of mature enamel crystals, with particular attention on the remineralization and regeneration of carious enamel fissure tissue.