ShEEP Request for a Perkin Elmer Quantum GX2 Micro CT Imaging System

通过此应用程序，我们向ORD共享设备评估计划申请资金 (绵羊)购买一台活体动物微型CT扫描仪，作为共享资源存放在 奥马哈退伍军人医学中心的研究服务。具体地说，我们寻求获得Perkin Elmer Quantum GX2 Micro CT高分辨率桌面活体显微断层摄影仪。Quantum GX2允许 用于活体啮齿动物物种的快速、高分辨率全身成像。Quantum GX2将提供 奥马哈退伍军人目前没有的最先进的活体动物成像能力 事务医学中心(VAMC)校园或其学术附属机构，内布拉斯加州医学大学 中心(UNMC)。该设备将为现有的奥马哈VA共享成像系统增加新的功能 设备包括之前由SHEEP奖购买的IVIS Lumina K系列III系统 以及用于身体成分测定的Echo MRI成像系统。因此，Quantum GX2微型CT成像系统将显著增强奥马哈和VA的研究能力 调查人员以及吸引与附属调查人员的新合作。Quantum GX2是一款 具有可变放大倍数的高性能台式活体微型CT扫描小 实验动物以及体外组织。该设备具有分辨率高、图像尺寸大、 圆形扫描和螺旋扫描重建，以及低剂量成像。因为大的 可对Quantum GX2进行图像视野、全身小鼠和大鼠扫描。这个 可变放大率允许以高空间分辨率扫描骨骼和组织样本 2.3微米像素大小。一系列过滤器允许不同的组织密度应用，从肺组织到 骨头。此外，Quantum GX2体内微型CT对动物的辐射剂量较低 允许多次纵向扫描，没有辐射引起的副作用。Quantum GX2 实现3D图像重建和组织形态测量的定量计算。 重要的是，Quantum GX2微型CT扫描仪针对纵向分析进行了优化，这是 直接适用于在OVAMC进行的研究，这些研究正在调查功能 以及与退伍军人管理局的使命直接相关的疾病的解剖学基础。《量子》 GX2 Micro CT成像系统具有灵活性，可以满足所有列出的实验需求 参与此申请的调查人员。这一成像系统为 奥马哈VAMC以及我们的附属大学(UNMC)，从而创造了新的能力， 当前不存在。Quantum GX2将允许以新的方式分析我们的研究人员现有的 与老兵相关的临床前疾病模型，从而促进对疾病的理解 病理生理学希望推进治疗干预措施，支持 弗吉尼亚州在推进今天退伍军人的医疗保健方面的作用。

With this application we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a live animal micro CT scanner as a shared resource to be housed in the Research Service of the Omaha VA Medical Center. Specifically, we seek to obtain a Perkin Elmer Quantum GX2 micro CT high-resolution desktop in vivo microtomograph. The Quantum GX2 allows for rapid, high-resolution full body imaging of live rodent species. The Quantum GX2 will provide state-of-the art in vivo live animal imaging capabilities not currently available on the Omaha Veterans Affairs Medical Center (VAMC) campus or its academic Affiliate, the University of Nebraska Medical Center (UNMC). This device will add new capabilities to the existing Omaha VA shared imaging equipment consisting of an IVIS Lumina K Series III system previously purchased by a ShEEP award as well as an Echo MRI imaging system for body composition determinations. As such, the Quantum GX2 micro CT imaging system will significantly enhance the research capabilities of both Omaha VA investigators as well as attract new collaborations with affiliate investigators. The Quantum GX2 is a high performance desktop in vivo micro-CT with variable magnification levels for scanning small laboratory animals as well as ex vivo tissues. This device features high resolution, large image size, both round and helical scanning for reconstruction, and low dose imaging. Because of the large image field of view of the Quantum GX2, full body mouse and rat scanning can be performed. The variable magnification allows scanning bone and tissue samples with high spatial resolution down to 2.3 µm pixel size. A range of filters allows for diverse tissue density applications from lung tissue to bone. Further, the Quantum GX2 in vivo micro CT administers low radiation dose to the animals allowing multiple longitudinal scans without radiation-induced side effects. The Quantum GX2 enables reconstruction of 3D-images and the quantitative calculation of tissue morphometry. Importantly, the Quantum GX2 micro CT scanner is optimized for longitudinal analyses, which is directly applicable to the studies being performed at the OVAMC that are investigating the functional and anatomical basis for diseases that are directly relevant to the mission of the VA. The Quantum GX2 micro CT Imaging System has the flexibility to serve the experimental needs of all the listed participating investigators in this application. This imaging system creates new capability for both the Omaha VAMC as well as our Affiliate University (UNMC), and thus creates new capacity that does not currently exist. The Quantum GX2 will allow for new ways of analyzing our researchers’ existing Veteran-relevant, preclinical disease models, thus promoting the understanding of disease pathophysiology in hopes to advance the therapeutic interventions that will support the mission of the VA in advancing the health care of today’s Veterans.