The Tissue Phenotyping and Bone Metabolism Core Facility

组织表型和骨代谢核心的总体目标，由经验丰富的研究人员博士领导。 Bouxsein和Demay，是提供肾脏和肌肉骨骼组织的最高质量评估， 以及血清和尿液生化，以最有效的方式。核心将为调查人员提供 获得最先进的技术，接受新器械使用培训（视情况而定）， 实验设计、方案开发、数据分析和解释。核心提供了层次化， 在具有遗传改变的啮齿动物模型中对肌肉骨骼表型进行多尺度评估，以及 饮食和药物干预，允许有效评估组织样本， 时间和资源。总的来说，核心小组在支持调查人员开展 项目方案，证明了所有项目所采用的核心，标本的高效处理， 以及核心为之提供稿件的数量。在这次更新中，核心将支持所有四个 拟议项目。在本供资期内，核心方案支助了所有项目，并将继续这样做 通过解决以下三个具体目标：1）提供最先进的血清和尿液 生化分析;组织学和组织形态学分析;骨骼成像和生物力学 2）促进一种具有成本效益的、有效的、综合的、分级的方法来评价组织 表型;和3）通过提供实践培训，研究设计方面的专家建议， 新方案/探针/抗体的故障排除以及核心机构生成结果的解释。 拥有经验丰富的领导和技术人员，以及先进的设备和严格的质量控制 为了实现这些目标，该中心将提供以下服务，包括： 技术;定量组织形态测定;体内骨密度测定和身体成分;高分辨率 通过微计算机断层扫描进行骨微结构测量;通过锇进行骨髓脂肪定量 四氧化物微计算机断层扫描;全骨和组织水平生物力学测试;以及血清和 与骨和肾代谢有关的尿生化。通过在核心区域内进行这些评价， 设施，高质量的控制标准可以很容易地保持。此外，核心将提供协调， 及时评估，并将根据需要协助研究者进行研究设计和结果解释。 核心人员的工资和基本用品由核心预算支付，但收取使用费 为某些服务提供资金，以抵消核心预算未涵盖的开支。我们发现这种混合方法， 薪金和基本用品由核心支付，但有些服务需要用户付费， （c）确保核心项目的运作，并在各项目之间公平地分享资源和分摊费用。

The overall goal of the Tissue Phenotyping and Bone Metabolism Core, led by experienced investigators Drs. Bouxsein and Demay, is to provide the highest quality assessment of kidney and musculoskeletal tissues, as well as serum and urine biochemistry, in the most efficient manner. The core will provide investigators with access to state-of-the-art technology, training in use of new devices (as appropriate) and consultation on experimental design, protocol development, data analysis and interpretation. The core provides hierarchical, multi-scale assessment of musculoskeletal phenotypes in rodent models with genetic alterations, as well as dietary and pharmacologic interventions, allowing the efficient evaluation of tissue samples, with regard to both time and resources. Altogether the core has been highly effective in supporting the investigators in the Program Project, as evidenced by the use of the core by all projects, the efficient processing of specimens, and number of manuscripts to which the core contributed. In this renewal, the core will support all four proposed projects. In the current funding period, the core supported all the projects and will continue to do so by addressing the following three specific aims: 1) Provide access to state-of-the-art serum and urinary biochemical analyses; histologic & histomorphometric analyses; skeletal imaging & biomechanical assessments; 2) Promote a cost-effective, efficient, integrated, hierarchical approach to evaluation of tissue phenotypes; and 3) Support investigators by providing hands-on training, expert advice on study design, trouble shooting of new protocols/probes/antibodies and interpretation of results generated by the Core facility. With experienced leadership and technical staff, as well as state-of-the-art equipment and strict quality control measures, the core will achieve these aims by offering the following services, including: a variety of histologic techniques; quantitative histomorphometry; in vivo bone densitometry and body composition; high resolution bone microarchitecture measurements via micro-computed tomography; marrow fat quantification via osmium tetraoxide micro-computed tomography; whole bone and tissue level biomechanical testing; and serum and urine biochemistry relating to bone and kidney metabolism. By conducting these evaluations within the core facility, high quality control standards can be readily maintained. Moreover, the core will provide coordinated, timely assessments, and will assist investigators with study design and interpretation of results, as needed. Salaries of core personnel and basic supplies are covered in the Core budget, however user fees are collected for some services to offset expenses not covered in the core budget. We find that this hybrid approach, where salaries and basic supplies are covered by the core, but some services require users fees, results in a stable operation of the Core, with equitable resource sharing and cost allocation amongst the projects.