Molecular Cytogenetics

项目摘要-分子细胞遗传学共享资源 分子细胞遗传学共享资源(MCSR)为制备人类和 适合于全基因组的分子遗传学和细胞遗传学分析的小鼠样本。我们的目标是 为AECC研究人员提供分析各种不同基因组所需的全套服务 分辨率级别。这些服务包括建立EBV转化的细胞系，以及浓缩 可以超低温保存并储存在液氮中的血细胞亚型。金黄色葡萄球菌DNA和mRNA的提取 还提供了各种组织培养样本以及初级活组织检查。完整的服务面板是 条件是，从中期染色体的制备到定制设计的探针和 用于检测拷贝数和结构染色体改变的杂交。MCSR提供 所有细胞遗传学水平的分析，从标准核型到光谱核型，以及从整体 染色体涂抹到基因组独特区域的特定位点的探针上。最近开发的一种靶向 现在还提供了用于看门人和看门人基因突变分析的测序小组来进行研究 癌症基因组的单核苷酸分辨率。MCSR人员接受培训，以设计有针对性的、轨迹- 对调查人员感兴趣的区域进行具体探测。所有探头都是内部定制设计和生成的。 MCSR还对石蜡包埋的组织进行杂交，以及图像采集和完整 数据分析。

PROJECT SUMMARY - MOLECULAR CYTOGENETIC SHARED RESOURCE The Molecular Cytogenetic Shared Resource (MCSR) provides tools for the preparation of human and murine samples suitable for molecular genetics and cytogenetics analysis of the entire genome. The goal is to provide AECC investigators with the whole spectrum of services necessary to analyze the genome at various levels of resolution. These services include the establishment of EBV transformed cell lines, and enrichment of blood cell subtypes that can be cryopreserved and stored in liquid nitrogen. Isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies is also provided. A full panel of services is provided, from preparation of metaphase chromosomes to the generation of custom designed probes and hybridization for the detection of copy number and structural chromosomal alterations. The MCSR provides analyses at all cytogenetic levels of resolution from standard to spectral karyotyping, and from whole chromosome paints to locus-specific probes for unique regions of the genome. A recently developed targeted sequencing panel for the mutational analysis of caretaker and gatekeeper genes is now also offered for studies at single nucleotide resolution of the cancer genome. MCSR personnel are trained to design targeted, locus- specific probes for regions of interest to investigators. All probes are custom designed and generated in-house. The MCSR also performs hybridizations on paraffin-embedded tissues as well as image acquisition and full data analysis.