Proteomics

摘要，蛋白质组学核心(PC) 印第安纳大学医学院(IUSM)蛋白质组学核心(PC)是以质谱学(MS)为中心的 蛋白质分析中心在亲和纯化质谱学方法和 定量蛋白质组学。国际宇航科学院最近购买了两台最先进的质谱仪，两台超高分辨率的 加压液相色谱系统和各种电离源，便于创新应用 为IUSM的所有研究人员，特别是IU Simon提供的基础和临床研究问题的蛋白质组学方法 癌症中心(IUSCC)成员。我们的中心目标是应用和开发最先进的蛋白质组学方法 衡量蛋白质生物学的全系统变化，因为它与癌症生物学问题有关。蛋白质组学 核心和IUSCC领导层制定了以下具体目标，以满足IUSCC的关键需求 蛋白质组学服务成员：具体目标1：为IUSCC用户提供最先进的蛋白质组学 以全球蛋白质组和磷酸蛋白质组图谱为重点的服务；具体目标2：发展领先的 在化学蛋白质组学中提供边缘蛋白质组学服务，以促进IUSCC的药物发现和药物表征； 特定目的3：扩大亲和纯化MS分析用于高置信度相互作用的鉴定 和具体目标4：发展、提供和/或组织与以下方面有关的教育机会 蛋白质组学技术、实验设计和数据分析。

ABSTRACT, Proteomics Core (PC) The Indiana University School of Medicine (IUSM) Proteomics Core (PC) is a mass spectrometry (MS)-centric protein analysis center with extensive expertise in affinity-purification mass spectrometry methods and quantitative proteomics. IUSM has recently purchased two state-of-the-art mass spectrometers, two ultra high- pressure liquid chromatography systems, and a variety of ionization sources to facilitate innovative application of proteomics methods to basic and clinical research questions for all researchers at IUSM, particularly IU Simon Cancer Center (IUSCC) members. Our central goal is to apply and develop state-of-the-art proteomics methods to measure system-wide changes in protein biology, as it relates to cancer biological questions. The proteomics core and IUSCC leadership have developed the following specific aims to meet the critical needs of IUSCC members for proteomics services: Specific Aim 1: To provide IUSCC users with state-of-the art proteomics services with a focus on global proteome and phosphoproteome profiling; Specific Aim 2: To develop leading edge proteomics services in chemoproteomics to facilitate drug discovery and drug characterization in IUSCC; Specific Aim 3: To expand affinity-purification MS analysis for identification of high-confidence interacting proteins; and Specific Aim 4: To develop, provide, and/or organize educational opportunities related to proteomics technologies, experimental design and data analysis.