Adapting molecular food safety tests to alternative matrices

项目总结/摘要 在一些国家，需要监测引起沙门氏菌病等肠道疾病的病原体， 这些部门包括食品和饲料生产、零售设施和兽医医院。几 可商购的试剂盒可用于此目的，但主要用于人 食品检测食品生产设施的全面监测计划应包括 能够测试多种类型的样品，包括患病或死亡动物的组织和粪便 和/或啮齿动物和环境拭子。其他样品类型的快速测定验证 例如动物组织、粪便样本和环境拭子， 全面的监测工作和疫情管理计划。 对沙门氏菌等肠道细菌病原体的快速筛查存在大量需求 spp.包括食品和饲料生产设施在内的一些部门。几种市 现有的试剂盒可用于此目的，但AOAC仅对人类食品测试进行了验证。 其他基质（如动物组织、粪便样本和 迫切需要环境表面拭子。为了实现这一目标，我们建议 以下具体目的：1）开发用于以下的市售分子诊断测定： 沙门氏菌属，e. coli和李斯特菌属（Listeria spp.）用于粪便样本、动物组织，以及 2）将这些测定转移到开放阵列平台，以便于 测试效率和可转移到其他实验室。 将食品快速检测试剂盒的验证扩展到其他动物相关基质， 由于我们在兽医诊断方面的专业知识， 通过提供全面的肠道病原体监测和爆发的新方法， 管理拟议研究的长期目标是促进传播 高通量，成本效益的方法，用于筛选多种样品类型的广泛 肠道病原体的范围。

Project Summary/Abstract Monitoring for pathogens causing enteric diseases such as salmonellosis is needed in a number of sectors including food and feed production, retail facilities, and veterinary hospitals. Several commercially available kits are available for this purpose, but are validated primarily for human food testing. A comprehensive monitoring plan for food production facilities should include the ability to test multiple types of samples, including tissues and feces from sick or dead animals and/or rodents and environmental swabs. Validation of rapid assays for additional sample types such as animal tissues, fecal samples, and environmental swabs is urgently needed as part of comprehensive surveillance efforts and outbreak management plans. There is a large demand for rapid screening for enteric bacterial pathogens such as Salmonella spp. in a number of sectors including food and feed production facilities. Several commercially available kits are available for this purpose, but are AOAC validated only for human food testing. Validation of rapid assays for additional matrices such as animal tissues, fecal samples, and environmental surface swabs is urgently needed. In order to accomplish this, we propose the following specific aims: 1) Validate commercially available molecular diagnostic assays for Salmonella spp., E. coli, and Listeria spp. for use in fecal samples, animal tissues, and environmental swabs; 2) Transfer these assays to an open array platform in order to facilitate testing efficiency and transferability to other laboratories. Extending validations of rapid food testing kits for additional animal-related matrices, which is feasible due to our expertise in veterinary diagnostics, will have direct relevance to public health by providing new methods for comprehensive enteric pathogen surveillance and outbreak management. The long-term goal of the proposed research is to facilitate the dissemination of high-throughput, cost-effective methods for screening of multiple sample types for a broad range of enteric pathogens.