iPSC Derived Cardiac Microchambers for Embryonic Drug Screening

 描述：在这项应用中，我们的目标是建立一种体外模型来概括早期心脏发育，用于妊娠期间常用的特定药物的安全性评估。在目标1中，我们将验证我们的假设，即可以通过人诱导多能干细胞(HiPSCs)的微图案化和分化来在体外模拟心脏组织的形态发生。我们将建立和优化我们的体外早期发育心脏模型，通过获得由不同几何形状的HiPSC模式生成的全光谱3D心脏微组织。在目标2中，我们将通过A类和X类妊娠风险药物作为阴性对照和阳性对照来验证我们的模型，因此我们将能够预测B-D类药物对心脏早期发育的毒性。

 DESCRIPTION: In this application, we aim to establish an in vitro model to recapitulate early heart development for safety assessment of specific drugs commonly administrated during pregnancy. In aim 1, we will test our hypothesis that cardiac tissue morphogenesis can be modeled in vitro by micro patterning and differentiation of human induced pluripotent stem cells (hiPSCs). We will establish and optimize our in vitro early developing heart model by obtaining a full spectrum of 3D cardiac micro tissues generated from hiPSC patterns with different geometries. In aim 2, we will validate our model by the drugs listed in pregnancy risk categories A and X as negative and positive controls, so we will be able to predict the drug toxicity from categories B-D on early heart development.