Development of membrane anchored cytokines as novel vaccine adjuvants for the elderly

承包商将使用IL-12和GM-CSF的工程版本作为佐剂。 IL-12和GM-CSF的工程化形式在羧基末端包括糖基磷脂酰肌醇（GPI）锚，其允许它们掺入脂质双层，例如流感病毒样颗粒（VLP）。 佐剂的开发将在老年人群中的流感背景下进行。 具体任务包括（1）体外和（2）体内建立免疫学活性谱，以评价佐剂的能力。

The contractor will use engineered versions of IL-12 and GM-CSF as adjuvants. The engineered version of IL-12 and GM-CSF include a glycosyl phosphatidylinositol (GPI) anchor at the carboxyl terminal which permits their incorporation into lipid bilayers such as influenza viral like particles (VLP). The development of the adjuvant will be in the context of influenza in elderly populations. The specific tasks include the (1) in vitro and (2) in vivo establishment of an immunological profile of activity to evaluate the capability of the adjuvant.