Liquid CO2 Flash Chromatography System

应用分离公司建议开发一种价格具有竞争力的液态二氧化碳闪存 将以高分辨率和快速分离化合物的层析系统 大幅降低有机溶剂消耗。每年一次的制药公司 在药物发现实验室合成数千种化合物，试图找到新的 商业化的药物分子。这些合成的化合物中的每一个都可能需要 许多反应和提纯步骤，以供进一步测试。使用的主要技术是 制药行业对这些化合物的提纯方法是闪蒸层析法，这是首选的方法 由于样品装载能力高，使用方便。不幸的是，现有的闪存 色谱仪器的峰分辨率较差，并且使用大量的 以有机溶剂为流动相进行分离。在药物化学实验室内， 闪蒸层析法是化学废物的主要来源。应用分居发展成 第一阶段成功地将液体二氧化碳作为 非极性有机溶剂流动相的替代物。仪器迅速分离 测试化合物具有更高的化合物分辨率、显著的溶剂减少和良好的 复合恢复。这项第二阶段建议的具体目的是：(1)重新设计 根据对仪器样机的评价，对仪器部件进行优化 可靠性、适用性和组装性。(2)重新设计软件以提高可用性，包括 计算机界面以及溶剂泵和二氧化碳软件模块的集成。(3) 开发可手动拧紧且易于使用的专有墨盒和容器 高级层析。(4)最后，构建了两个优化的液体闪蒸色谱仪 在最终设计锁定之前，在生物技术和药物发现实验室进行Beta测试。

Applied Separations Inc. proposes to develop a competitively priced liquid CO2 flash chromatography system that will rapidly separate compounds with high resolution and significantly reduce organic solvent consumption. Pharmaceutical companies annually synthesize thousands of compounds in drug discovery laboratories in an attempt to find new drug molecules for commercialization. Each of these synthesized compounds may require many reaction and purification steps for further testing. The primary technique used in the pharmaceutical industry to purify these compounds is flash chromatography and it is preferred because of high sample loading capacity and ease of use. Unfortunately, existing flash chromatography instrumentation suffers from poor peak resolution and uses large volumes of organic solvents as the mobile phase in the separation. Within medicinal chemistry laboratories, flash chromatography is the main source of chemical waste. Applied Separations developed a Phase I prototype liquid CO2 flash chromatography system that successfully used liquid CO2 as a replacement for non polar organic solvent mobile phases. The instrument rapidly separated test compounds with increased compound resolution, significant solvent reduction and good compound recoveries. The specific aims of this Phase II proposal are to: (1) redesign the instrument based on the evaluation of the prototype and optimize the instrument components for reliability, serviceability, and assembly. (2) redesign software to improve usability including the computer interface and the integration of the solvent pump and CO2 software modules. (3) develop proprietary cartridges and vessels that are hand tightened and easy to use with superior chromatography. (4) Finally, construct two optimized liquid flash chromatographs for beta testing at biotechnology and drug discovery laboratories before the final design is locked.