NARMS: Enhance and Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens

项目总结： 明尼苏达州卫生部(MDH)一直在与明尼苏达州卫生部合作 美国农业部(MDA)、美国疾病控制与预防中心(CDC)和美国食品和药物管理局(FDA)自2002年开始对零售食品进行研究。这 该项目旨在确定肠道中抗菌素耐药性的流行率 在零售肉类(包括鸡肉、猪肉、碎牛肉和碎火鸡)中发现的病原体。在……里面 明尼苏达州，MDH和MDA正在培养所有的肉类沙门氏菌，鸡肉和磨碎的火鸡肉 对弯曲杆菌来说。所有的分离株都进一步用MDH或MDA进行表征，并被送往FDA进行 进一步分析(包括培养确认、抗菌药敏感性测试和 分子亚型)。从历史上看，对40个样本(每种肉类商品10个)进行了检测 月份。2015年5月，在额外资金的支持下，我们能够增加样本数量 每月共抽取80份鸡肉及火鸡末样本(包括猪肉及 牛肉)。在这些样本中，所有80个样本都进行了沙门氏菌检测，所有20个火鸡样本和20个(一半)火鸡样本都进行了沙门氏菌检测 鸡肉样本进行了弯曲杆菌检测。明尼苏达州，2002年至2015年，1920年第184号 鸡肉样本(9.6%)，1760份火鸡末样本中222份(12.6%)，1680份猪肉样本中10份(0.6%) 1680个碎牛肉样本中有10个(0.6%)沙门氏菌检测呈阳性。也在 明尼苏达州2002-2015年，1760份鸡肉样本中有474份(26.9%)，1760份鸡肉样本中有38份(2.2%) 火鸡样本的弯曲杆菌检测呈阳性。2002-2007年间，720头猪肉中有1头(0.1%) 在720个碎牛肉样本中，0个样本的弯曲杆菌检测呈阳性。MDH和丙二醛 计划继续我们的合作安排。我们将从七个县采集样本 明尼阿波利斯--圣彼得堡保罗大都市区，占明尼苏达州人口的一半以上。 这项研究为我们对该物种、血清型和 在各种零售肉类中发现的弯曲杆菌和沙门氏菌的抗药性类型。 这些数据有助于指导我们对肉类可能存在的地方进行的疫情调查 人类的感染源。我们几乎没有遇到什么障碍或挑战 这项研究的经验和感觉一直是一个有价值的工具，增加了一个关键的组成部分 明尼苏达州的抗菌素耐药性图景。

Project Summary: The Minnesota Department of Health (MDH) has been working with the Minnesota Department of Agriculture (MDA), CDC, and FDA on the Retail Food Study since its inception in 2002. This project was designed to determine the prevalence of antimicrobial resistance among enteric pathogens found in retail meats (including chicken, pork, ground beef, and ground turkey). In Minnesota, MDH and MDA are culturing all meats for Salmonella and chicken and ground turkey for Campylobacter. All isolates are further characterized by MDH or MDA and sent on to FDA for further analysis (including culture confirmation, antimicrobial susceptibility testing, and molecular subtyping). Historically, 40 samples (10 from each meat commodity) were tested each month. In May, 2015, with additional funding, we were able to increase the number of samples of chicken and ground turkey for a total of 80 meat samples per month (including pork and beef). Among the samples, all 80 are tested for Salmonella, all 20 turkey samples and 20 (half) of the chicken samples are tested for Campylobacter. In Minnesota, from 2002-2015, 184 of 1920 (9.6%) chicken samples, 222 of 1760 (12.6%) ground turkey samples, 10 of 1680 (0.6%) pork samples, and 10 of 1680 (0.6%) ground beef samples tested positive for Salmonella. Also in Minnesota from 2002-2015, 474 of 1760 (26.9%) chicken samples and 38 of 1760 (2.2%) ground turkey samples tested positive for Campylobacter. During 2002-2007, 1 of 720 (0.1%) pork samples, and 0 of 720 ground beef samples tested positive for Campylobacter. MDH and MDA plan to continue our cooperative arrangement. We will collect samples from the seven-county Minneapolis-St. Paul metropolitan area, which comprises over half of Minnesota’s population. This study has contributed substantial data to our understanding of the species, serotypes, and antimicrobial resistance types of Campylobacter and Salmonella found in various retail meats. This data has been beneficial in guiding our outbreak investigations where meats may have been the source of infection in humans. We have encountered very few barriers or challenges in our experience with this study and feel it has been a valuable tool, adding a critical component to the picture of antimicrobial resistance in Minnesota.