Mechanisms of Breast Development and Carcinogenesis

组织和病理核心B由乳腺病理学家安德里亚·理查森博士指导。这个共享的资源核心将提供每个ER和HER2免疫表型的新鲜人类乳腺肿瘤样本，用于与布朗博士合作产生早期传代的可移植的人在小鼠体内的异种移植。组织和病理核心将描述异种移植瘤的特征，并在组织形态、免疫表型和分子(基因表达和突变)图谱方面与配对的原始人类肿瘤进行比较。这些异种移植模型将用于项目1中提出的研究， 2、4和6。该核心将提供来自非癌症妇女(乳房缩小)和具有生殖系BRCA1或BRCA2突变的妇女的新鲜乳腺组织，用于分离项目2和5中建议的研究的上皮祖细胞。收集的组织来自于对我们的研究库方案之一提供知情同意的妇女，该方案允许从临床样本中收集多余的组织用于研究，并收集长期结果数据并与其联系。此外，核心将提供研究病理学服务，以执行项目3和4中的小鼠肿瘤模型的组织病理学分析。

The Tissue and Pathology core B is directed by breast pathologist, Dr. Andrea Richardson. This shared resource core will provide fresh human breast tumor samples of each of the ER and HER2 immunophenotypes for generation of early passage transplantable human-in-mouse xenografts in collaboration with Dr. Brown. The tissue and pathology core will characterize the xenograft tumors and compare to the paired original human tumor for histomorphology, immunophenotype, and molecular (gene expression and mutation) profiling. These xenograft models will be used for studies proposed in projects 1, 2, 4 and 6. The core will provide fresh breast tissue from women without cancer (breast reductions) and from women with germline BRCA1 or BRCA2 mutation for isolation of epithelial progenitor cells for studies proposed in projects 2 and 5. The tissues collected are from women who provided informed consent to one of our research banking protocols that allows for collection of excess tissues from clinical specimens for research and for collection of and linkage to long term outcome data. In addition, the core will provide research pathology services to perform histopathology analysis of mouse tumor models in projects 3 and 4.