Tumorigenic effects of BRCA2 mutation and genomic instability in dogs

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Mammary tumors are the most common tumor type in both human and canine females. In women, carriers of mutations in BRCA2, a tumor suppressor gene product, have a higher risk of breast cancer. Canine BRCA2 has also been suggested to have a relationship with mammary tumors. However, clearly deleterious BRCA2 mutations have not been identified in any canine mammary tumors, and effects of BRCA2 mutations on tumorigenicity and genomic instability in dogs are unknown.In this study, 1. a PCR analysis method for canine BRCA2 was established, 2. effects of the missense mutations in canine BRCA2 on BRC repeats were elucidated, 3. tumour suppressor function of canine REIC/Dkk-3 in mammary gland tumors was analyzed.