[ShEEP/LAMb] Request for a Seahorse Extracellular Flux Analyzer

本申请中要求的仪器是SeaHorse细胞外通量分析仪套装 由6口井和96口井平台组成，适合进行先导研究和更大规模的高通量研究， 分别进行了分析。这些仪器是由海马生物科学公司制造的。购买价格 包括现场培训和一年保修。数据分析软件可从 制造商免费提供，可安装在不限数量的工作站上。这个 仪器通过氧气浓度和pH值随时间的变化来测量细胞呼吸 并能够辨别细胞呼吸中线粒体或糖酵解的部分。在……里面 此外，药物操作允许人们测量线粒体和糖酵解储备。 容量和基线细胞呼吸。使用额外的试剂，人们可以测量出 可归因于碳水化合物和脂肪代谢的细胞呼吸作用。这些测量结果是 使用浸泡在细胞培养液和预混合试剂中的两个独立的传感器获得 从制造商处购买。这款仪器设计成便于个人使用， 没有接受过代谢组学方面的专门培训，是衡量代谢的事实上的标准 活细胞中的参数。测量可以从培养的细胞系、原代培养物或 组织切片。测量时必须使用专用的一次性塑料盘子。单元格或 组织被放置在这些平板上的孔中，并可以类似地保持在细胞培养孵化器中 到常规的细胞培养系统。JJPVA没有同等的仪器。使用最接近的 位于我们位于西奈山的伊坎医学院学术分支机构的海马平台，并不是 这是可行的，因为将细胞培养物运输到相隔10英里的地方是有挑战的 机构。如果获得资金，该应用程序将为JJPVA调查人员提供访问黄金标准的机会 细胞代谢组学测量。该仪器将用于退伍军人管理局资助的下列领域的项目 阿尔茨海默病、创伤性脑损伤、艾滋病毒致病机制和脊髓损伤。这台仪器 将增加对细胞和培养细胞中细胞代谢的系统研究能力， 从而大大拓宽和加强对影响健康的医疗条件的了解 受这些疾病和残疾影响的退伍军人的健康和生活质量。

The instrument requested in this application is a Seahorse Extracellular Flux Analyzer package consisting of 6 well and 96 well platforms suitable for pilot studies and larger high throughput studies, respectively. These instruments are manufactured by Seahorse Bioscience. The purchase price includes on-site training and a one-year warranty. Data analysis software is available from the manufacturer for no charge and may be installed on an unlimited number of workstations. The instrument measures cellular respiration through changes over time on oxygen concentration and pH and is able to discern the portion of cellular respiration attributable to mitochondria or glycolysis. In addition, pharmacological manipulations permit one to measure mitochondrial and glycolytic reserve capacity and baseline cellular respiration. Using additional reagents, one can measure the fraction of cellular respiration attributable to metabolism of carbohydrates and fats. These measurements are obtained using two, separate sensors that are immersed in cell culture fluid and pre-mixed reagents purchased from the manufacturer. The instrument is designed to be easily used by individuals who do not have specialized training in metabolomics and is the de-facto standard for measuring metabolic parameters in living cells. Measurements may be obtained from cultured cell lines, primary cultures or tissue sections. Specialized, disposable plastic plates must be used for the measurements. Cells or tissues are placed into wells on these plates and can be maintained in cell culture incubators similarly to routine cell culture systems. No equivalent instrument is available at JJPVA. Use of the closest Seahorse platform, located at our academic affiliate the Icahn School of Medicine at Mount Sinai, is not feasible because of the challenges of transporting cell cultures over the 10 miles that separate the institutions. If funded, this application would afford JJPVA investigators access to the gold standard for cell metabolomics measurements. The instrument will be used in VA-funded projects in the fields of Alzheimer's disease, traumatic brain injury, HIV pathogenesis, and spinal cord injury. The instrument would add the capacity for systematic study of cellular metabolism in cells and cultured cells and, thereby, substantially broaden and enhance the understanding of medical conditions that affect the health and quality of life of veterans impacted by these diseases and disabilities.