Non-coding RNA & Bioinformatics Core

该项目的目的是显著提高我们对(1)家族性高胆固醇血症和(2)慢性肾脏疾病中HDL功能障碍机制的理解，以及(3)氧化修饰对HDL的影响。提出的研究策略是高度跨学科的，需要广泛的下一代RNA测序和转录组级分析。一个有效的、专门的非编码RNA和生物信息学核心是研究成功的关键。具体来说，非编码RNA和生物信息学核心旨在处理项目1、2和3的大量测序需求和数据分析。我们训练有素，技术娴熟，并在smRNA-Seq上发表。和RNA测序。此外，我们已经开发了内部测序分析管道，将由计算生物学家和生物统计学家在核心领导者Dr. Kasey C. Vickers的指导下实施。非编码RNA和生物信息学核心的目的是通过高质量的数据管理和集成到复杂的生物信息学系统中，及时有效地使用大量平行DNA测序来分析与HDL功能障碍相关的小RNA和长RNA表达和丰度。

The purpose of this program is to significantly advance our understanding of mechanisms of HDL Dysfunction in (1) Familial Hypercholesterolemia and (2) Chronic Kidney Disease, as well as the impact of (3) Oxidative modifications to HDL. The proposed research strategies are highly cross disciplinary and require extensive next-generation RNA sequencing and transcriptome-scale analysis. An effective and dedicated non-coding RNA and bioinformatics core is essential for the success of the proposed studies. Specifically, the Non-Coding RNA and Bioinformatics Core is designed to handle the large volume of sequencing needs and data analysis for Projects 1, 2, and 3. We are highly trained, skilled, and published in smRNA-Seq. and RNA seq. Furthermore, we have developed in-house sequencing analysis pipelines that will be implemented by computational biologists and biostatisticians under the guidance of the Core Leader, Dr. Kasey C. Vickers. The purpose of the Non-Coding RNA and Bioinformatics Core is to use massive parallel DNA sequencing to analyze small and long RNA expression and abundance associated with HDL dysfunction in a timely and efficiently manner with high-quality data management and integration into complex bioinformatics systems.