National Resource for Translational and Developmental Proteomics

 描述(由申请人提供)：在这里，我们提出了一个国家翻译和发育蛋白质组学资源(NRTDP)。在八个推动生物医学项目中概述了科学驱动因素，这些项目激励了四个技术研究和开发项目的具体目标。这些联合活动致力于改进以整个蛋白质为主要测量单位的基于质谱学的蛋白质组学，其影响将是巨大的。综合了资源的所有方面(包括生物医学应用、技术开发、传播和培训)，预测的影响与P41生物医学技术研究资源的精神和文字以及NIH作为一个整体的战略目标保持一致。将创新应用于临床和转译研究的不同领域的及时挑战，将确保我国在“下一代”蛋白质组学技术方面的领先地位。这些技术将服务于推动项目和研究人员，以提高科学理解和诊断，最终改善癌症患者、肾移植接受者和患有冠心病的患者的生活，并保护癌症幸存者(肿瘤生育)的生育能力。更具体地说，我们将把超高性能蛋白质组学的重点放在各种癌症的研究样本集上，包括慢性淋巴细胞白血病、多发性骨髓瘤和急性髓系白血病。在技术方面，我们将加快开发领先的自上而下蛋白质组学平台，包括两种新的傅立叶变换仪器、新的蛋白质分级技术和Autopilot软件套件，使生物医学项目的战略目标能够管理日常数据收集的最详细方面。一个量身定做的工作流程，能够对核心组蛋白进行全面和详尽的分析，进一步增加了这一高度协作和恭敬的国家资源中可用的技术组合，该资源拥有一支表现突出的高绩效技术和管理团队。很好的证据表明，该团队有能力将高端研究活动提炼成强大的服务线。 对实验室作为培训中心的兴趣一直很浓厚(迄今已有十几名访问学者)，并列入了扩大这些活动的具体计划。申请书中还描述了信息和技术传播的几种途径。所有这些活动的管理和执行都得到了西北大学蛋白质组学卓越中心(PCE)成熟的高级团队的保证，该中心也拥有非常强大的机构支持，以使芝加哥正在进行的蛋白质组学与NIH资助的生物和翻译研究的国家优先事项保持一致。NCI、NICHD、NIDDK、NIGMS和NHLBI等几个机构将对这里设想的普通医学科学的进步感兴趣，所有这些机构都在蛋白质组学研究上投入了大量资金。我们在蛋白质组学卓越中心的高级研究人员团队，以及全国各地十几个合作实验室，期待着对拟议的NRTDP的反馈。

 DESCRIPTION (PROVIDED BY APPLICANT): Here we propose a National Resouce for Translational and Developmental Proteomics (NRTDP). The science drivers are outlined in eight Driving Biomedical Projects that motivate the specific aims for four Technology Research and Development Projects. The impact of these combined activities, devoted to improving mass spectrometry-based proteomics using whole proteins as the primary unit of measurement, will be substantial. Integrated over all aspects of the resource (including biomedical application, technology development, dissemination and training), the projected impacts are aligned with both the spirit and the letter of a P41 Biomedical Technology Research Resource and the strategic goals of the NIH as a whole. Applying innovation to timely challenges in diverse areas of clinical and translational research will secure a leadership position for our nation in 'next-generation' proteomics technology. These technologies will serve driving projects and investigators to improve scientific understanding and diagnostics, ultimately improving the lives of cancer patients, recipients of kidney transplants, those afflicted with coronary heart disease, and to preserve fertility in women survivors of cancer (oncofertility). More specifically, we will focus ultra-high performance proteomics on sample sets deriving from research on various cancers, including chronic lymphocytic leukemia, multiple myeloma, and acute myeloid leukemia. In technology, we will accelerate the development of the leading platform for top-down proteomics, including two new Fourier-Transform instruments, new protein fractionation technologies, and the AUTOPILOT software suite to allow strategic objectives of biomedical projects to govern the most detailed aspects of data collection on a daily basis. A tailored workflow to enable pervasive and exhaustive analysis of core histones further augments the technology portfolio available in this deeply collaborative and deferential national resource housing a demonstrably high performing technical and administrative team. Good evidence of the team's ability to distill high-end research activities into robust servce lines is now visible. Interest in the laboratory as a hub for training has been robust (with over a dozen visiting scholars to date) and specific plans for expansion of these activities are included. Several routes of information and technology dissemeniation also described in the application. The administration and execution of all these activities is assured by a proven senior team operating the Proteomics Center of Excellence (PCE) at Northwestern, which also has very strong institutional backing to align proteomics ongoing in Chicago with national priorities of NIH-funded biological and translational research of the NIH. The advancement of general medical science envisioned here will be of interest to several institutes including NCI, NICHD, NIDDK, NIGMS, and NHLBI - all of which are heavily invested in proteomics research. Our team of senior researchers at the Proteomics Center of Excellence, along with over a dozen collaborating laboratories around the country, look forward to feedback regarding the proposed NRTDP.