Driving Biomedical Projects

推动生物医学项目 摘要 国家翻译和发育蛋白质组学资源（NRTDP）将与 来自不同地理位置的研究人员将完成八个寻求进步的生物医学项目（DBP） 这需要在生物医学研究的四大支柱中进行自上而下的蛋白质组学研究。虽然这些项目来自不同的 在科学和医学领域，每个项目都不能用现有的技术进行优化。 时间点这些项目推动了 NRTDP前瞻性-为他们提供一个有形和可量化的目标，其总体目标是影响和改善 人体健康我们的研发项目专注于每个DBP进展过程中的特定技术， 加速自上而下的稳健性，以创新特定细胞的新型靶向和发现方法 类型整合每个研发项目的部分内容，我们围绕四个支柱安排了八个DBP（描述如下 在整体部分中有详细说明）： 具体目标1（支柱1）：癌症蛋白质组学。p53、RAS和H3.3K27M的自顶向下蛋白质组学。 具体目标2（支柱2）：免疫蛋白质组学。ESKAPE多重耐药病原体的自上而下分析 以及使用定量自上而下蛋白质组学鉴定移植排斥标记物。 具体目标3（支柱3）：神经蛋白质组学。特别关注脑癌和蛋白质病， 神经变性和衰老。 具体目标4（支柱4）：心脏蛋白质组学。鉴定与ApoC-III蛋白质型相关的所有谱 冠状动脉疾病 正是通过下一代蛋白质组学技术的综合开发，我们将能够 在这四个支柱内推动生物医学项目取得成功。随着这些项目的进展 在赠款的整个生命周期内，它们将被重新评估，一旦技术成熟， 转化为协作和服务项目，允许新的生物医学问题驱动下一个 一代技术。

Driving Biomedical Projects Abstract The National Resource for Translational and Developmental Proteomics (NRTDP) will collaborate with geographically diverse investigators to complete eight Driving Biomedical Projects (DBPs) seeking advances that require top-down proteomics in four pillars of biomedical research. While these projects arise from disparate areas of science and medicine, each project cannot be performed optimally with the technology available at this point in time. These projects drive the Technology Research and Development (TR&D) projects of the NRTDP forward – giving them a tangible and quantifiable goal with the overarching goal to impact and improve human health. Our TR&D projects focus on specific technologies throughout the progression of each DBP from accelerating top-down robustness to innovating novel targeted and discovery approaches from specific cell types. Integrating parts of each TR&D project, we have arranged our eight DBPs around four pillars (described in detail in the Overall section): Specific Aim 1 (Pillar 1): Cancer Proteomics. Top-down proteomics of p53, RAS, and H3.3K27M. Specific Aim 2 (Pillar 2): Immunoproteomics. Top-down analysis of multi-drug resistant ESKAPE pathogens and identification of transplant rejection markers using quantitative top-down proteomics. Specific Aim 3 (Pillar 3): Neuroproteomics. A specific focus on brain cancer and proteinopathies with roles in neurodegeneration and aging. Specific Aim 4 (Pillar 4): Cardiac Proteomics. Identifying the repertoire of ApoC-III proteoforms associated with coronary artery disease. It is through the integrated development of next generation proteomics technologies that we will be able to achieve success in Driving Biomedical Projects within these four pillars. As these projects progress throughout the lifetime of the grant, they will be reassessed and once technologies are matured, may be translated into Collaboration and Service projects, allowing new biomedical problems to drive the next generation of technology.