Polymer Sensors for Rapid Cell Genotyping

在这份提案中，Vuronix Technologies与马萨诸塞州大学的文斯·罗特洛教授合作 Amherst的目标是开发一种用于验证细胞系的原型试剂盒。细胞系有很大的范围 在学术和生命科学行业的应用，包括基因组学、药物 发现、组织工程、再生医学和毒性测试。然而，细胞培养 容易受到外来细胞的污染，这可能会迅速取代原始细胞。这个 培养细胞的身份应该例行验证，但大多数实验室不进行监测 它们的细胞系的身份，许多细胞系被错误地识别。成本、精力和时间 需要确认细胞系的身份一直是采用细胞系的障碍 将鉴定作为一种常规质量控制措施 我们的初步结果表明，我们的基于模式的方法能够区分 无论是细胞基因型还是表型。我们的研究将集中在开发一种‘极简主义’聚合物- 唯一可以快速区分细胞类型和基因/表型的传感器阵列。这个 聚合物平台提供了一个可扩展和可重现的平台，这一优势将 通过罗特洛和武罗尼克斯之间的密切合作，进一步推动了这一进程。第一阶段的目标 是开发基于聚合物纳米颗粒的细胞传感策略，并针对一系列 单元类型。这项提议与美国国立卫生研究院促进创造性发现的使命直接相关 人类福祉，以及NIGMS了解生物过程和 推进疾病诊断、治疗和预防。

In this proposal, Vuronyx Technologies in collaboration with Prof. Vince Rotello at UMass Amherst aims to develop a prototype kit for validation of cell lines. Cell lines have a wide range of applications in both academic and life sciences industries, including genomics, drug discovery, tissue engineering, regenerative medicine, and toxicity testing. However, cell cultures are prone to contamination by foreign cells, which may rapidly displace the original cells. The identity of cultured cells should be routinely verified, but a majority of laboratories do not monitor the identity of their cell lines, and many cell lines are misidentified. The costs, effort and time required to confirm the identity of cell lines has been a barrier to adoption of cell line identification as a routine quality control measure Our preliminary results show that our pattern-based approach is capable of distinguishing both cell genotype and phenotype. Our research will focus on developing a ‘minimalist’ polymer- only sensor array that can rapidly discriminate between cell types and geno/phenotypes. The polymer platform presents a scalable and reproducible platform, an advantage that will be furthered through close collaboration between Rotello and Vuronyx. The objective in Phase 1 is to develop the polymer nanoparticle based cell sensing strategy and test it against a range of cell types. This proposal is directly relevant to NIH’s mission of fostering creative discoveries for human well-being, as well as NIGMS’s mission of understanding of biological processes and advancing disease diagnosis, treatment and prevention.