Integrated Hydrogel Biosensor and Wireless Readout for Real-Time Salivary Cortisol Monitoring

项目总结 皮质醇测定是诊断心理压力和慢性病的可靠方法 与皮质醇紊乱有关。荷尔蒙皮质醇在紧张和烦躁的状态下会在体内释放。 与创伤后应激障碍等相关心理疾病有较高的相关性。唾液 皮质醇水平是反映血浆游离皮质醇水平的良好指标。皮质醇水平通常会波动 全天，皮质醇相关疾病的诊断和心理压力水平的评估 不能依赖单一的测量；通过绘制皮质醇水平随时间的变化来确定趋势是必要的 表明心理压力开始出现或被诊断为疾病。目前的皮质醇测量技术有 以实验室为基础和间歇性的，不能用于连续监测。我们计划创造一种可穿戴的，在- 口腔传感器，支持唾液皮质醇的实时动态监测。该设备利用新的 由生物响应水凝胶和嵌入的导电纳米线网络组成的生物传感器体系结构。 该生物传感器的检测下限为0.2 nm，响应时间小于5 分钟，根据实时检测应用程序的要求。 建议的策略实现了对中性带电皮质醇的敏感、特异和可逆检测，而不需要 加化学试剂或氧化还原试剂进行信号转导。这样的能力克服了限制 在电荷型晶体管/纳米线生物传感器或电化学技术中遇到。与 定制安培读出集成电路(IC)将提供高分辨率、定量传感器 在毫米级微芯片上进行测量并增加蓝牙低能量(BLE)无线收发器 将向智能手机提供实时数据传输，以进行处理、存档和上传到云中。

PROJECT SUMMARY Measurement of cortisol is a reliable approach to diagnosing psychological stress, as well as chronic diseases related to cortisol disorders. The hormone cortisol is released in the body during stressed and agitated status and has a high correlation with post-traumatic stress disorders and other related psychological diseases. Salivary cortisol level is an excellent index of the unbound plasma free cortisol. Cortisol level typically fluctuates throughout the day, and diagnosis of cortisol-related diseases and assessment of psychological stress level cannot rely on a single measurement; mapping cortisol levels over time through is necessary to identify trends indicative of psychological stress onset or diagnosis of disease. Current cortisol measurement techniques are lab-based and episodic, and cannot be used for continuous monitoring. We propose to create a wearable, in- mouth sensor that supports ambulatory monitoring of salivary cortisol in real time. The device leverages a new biosensor architecture consisting of a bioresponsive hydrogel and embedded conductive nanowire networks. The proposed biosensor is expected to achieve a detection limit of 0.2 nM with a response time of less than 5 minutes, as required for real-time detection applications. The proposed strategy enables sensitive, specific, and reversible detection of neutrally-charged cortisol without the addition of chemical reporters or redox reagents for signal transduction. Such capability overcomes the limits encountered in charge-based transistor/nanowire biosensors or electrochemical techniques. Integration with a custom amperometric read-out integrated circuit (IC) will provide high-resolution, quantitative sensor measurement in a mm-scale microchip and the addition of a Bluetooth low energy (BLE) wireless transceiver will provide real-time data transmission to a smartphone for processing, archiving, and upload to the cloud.