Mouse Core

核心B。鼠标核心 项目摘要 小鼠核心，核心B，被设计为向三个实验动物提供野生型和突变型小鼠睾丸。 此应用程序中的项目（Mello、Theurkauf和Zamore）。辛迪·蒂平，谁将工作人员的核心，是熟练的 基因分型和育种小鼠，她将跟踪研究人员的小鼠需求使用mLIMS，安全的 Zamore实验室目前使用的基于云的菌落管理工具。最初，Tipping女士将提供 新鲜解剖的睾丸给本申请项目的研究人员。随后，Zamore博士， Mouse Core的负责人，将在监管机构中为各个研究者实验室的成员提供建议。 和技术程序所需的工作与小鼠，使他们能够被添加到Zamore实验室的 现有IACUC批准的方案。Tipping女士将指导这些研究人员使用标准的小鼠方法， 包括睾丸解剖重要的是，Mouse Core将采用条形码系统进行样本管理， 开发一个LIMS数据库，该数据库将与生物信息学核心（核心A）接口，并建立严格的质量管理体系 控制措施。研究者可通过任何网络浏览器访问这些受密码保护的数据。这些 核心活动将促进研究项目与本计划项目核心之间的整合 相关性 鼠标核心（Core B）提供的服务对于支持基础研究至关重要 三个实验项目中的每一个。鼠标核心将整合项目在他们的努力 了解piRNA在哺乳动物睾丸中的作用，促进发现进化上保守的 以及皮尔纳途径的拟南芥特异性特征。

Core B. Mouse Core Project Summary The Mouse Core, Core B, is designed to provide wild-type and mutant mouse testes to the three experimental Projects in this application (Mello, Theurkauf, and Zamore). Cindy Tipping, who will staff the core, is skilled in both genotyping and breeding mice, and she will track investigator's mouse needs using mLIMS, the secure cloud-based colony management tool in current use by the Zamore laboratory. Initially, Ms Tipping will provide freshly dissected testes to the researchers from the Projects in this application. Subsequently, Dr. Zamore, the director of the Mouse Core, will advise members of the individual investigators' laboratories in the regulatory and technical procedures required to work with mice, allowing them to be added to the Zamore laboratory's existing IACUC-approved protocol. Ms Tipping will instruct these researchers in standard mouse methods, including testis dissection. Importantly, the Mouse Core will adopt a barcode system for sample management, develop a LIMS database that will interface with the Bioinformatics Core (Core A), and institute rigorous quality control measures. Investigators will have access to this password-protected data via any web browser. These Core activities will promote integration between the research projects and cores of this Program Project Relevance The services provided by the Mouse Core (Core B) are essential to support the fundamental research proposed by each of the three experimental Projects. The Mouse Core will integrate the Projects in their efforts understand the role of piRNAs in the mammalian testis, promoting the discovery of evolutionarily conserved and mammalian-specific features of the piRNA pathway.