ShEEP Request for Acquisition of Sable Promethion Caging

摘要：我们通过共享设备评估计划(SHEEP)申请资金 研究和发展办公室提供的研究设备管理程序供购买 8笼小鼠和8笼大鼠连续笼养代谢表型系统(Sable 系统，拉斯维加斯，内华达州，美国)，以扩大我们目前的扑美辛小鼠和大鼠系统。通过利用 连续的气流采样，允许对所有笼子进行每秒快速新陈代谢测量 同时，我们能够收集同步的新陈代谢和行为(运动、食物摄入量、水分 进气、车轮运行活跃度)措施。此外，新升级的系统将允许 环境传感器阵列，持续监测每个住房房间环境的变化 (温度、噪音、光线等)。这些设备将安置在明尼阿波利斯VAHCS研究中心内 军种兽医分队。额外的系统将允许对更多的动物进行研究 同时，使更快地完成控制和测试范例成为可能，并减少 控制组和试验组之间的可变性。这反过来将允许所有用户的实验数据的更大吞吐量 设计，提高明尼阿波利斯VAHCS调查员的生产率。该设备的使用将 补充现有的基于多用户的仪器库存，最终目标是提高效率 支持和支持翻译性退伍军人管理局研究。凯瑟琳·科茨博士将监督 在明尼阿波利斯VAHCS主任Patrick J.Kelly先生的全力支持下，设备和研究与 发展委员会主席罗纳德·巴赫博士。此设备的主要和次要用户组跨度 多个退伍军人研究学科，代表着以下领域的基础研究和转化性研究：老龄化， 心血管、癌症、神经科学、干细胞疗法和肥胖。退伍军人管理局资助的用户总数将为 使用了至少75%的仪器容量。次要用户组将占据25%-30%的 仪器容量。明尼阿波利斯VAHCS的能力继续保持在最大和最 VAHCS中的积极研究计划取决于其调查人员获得最新数据的能力 新一代高灵敏度、高分辨率仪器。收购扑灭净连续笼具 所需的代谢表型系统将加速和提高来自 多个学科可以获取、分析、显示和理解数据，并以受益为长期目标 美国退伍军人独特的医疗保健需求。这不仅有利于VAHCS研究的进展 但这将使所有美国人的医疗保健受益。

Abstract: We are requesting funds through the Shared Equipment Evaluation Program (ShEEP) under the Research Equipment Management program offered by the Office of Research and Development for purchase of an 8 cage mouse and an 8 cage rat Promethion continuous cage metabolic phenotyping systems (Sable Systems, Las Vegas NV,USA) to expand upon our current Promethion mouse and rat systems. By utilizing continuous air-flow sampling, which allows for rapid metabolic measurements every second for all cages simultaneously, we are able to collect synchronous metabolic and behavioral (movement, food intake, water intake ,wheel running activity) measures. In addition, the new upgraded systems will allow for an Environmental Sensor Array to continuously monitor changes in each of the housing room's environment (temperature, noise, light, etc). The equipment will be housed within the Minneapolis VAHCS Research Services Veterinary Medical Unit. The additional systems will allow for the study of more animals simultaneously, making it possible to complete control and test paradigms more quickly, and reducing variability between control and test groups. This in turn will allow greater throughput of all users' experimental designs, increasing the productivity of the Minneapolis VAHCS investigators.The use of this equipment will compliment an existing inventory of multiuser based instruments with the end goal of increasing the efficiency of and supporting translational VA research. Dr. Catherine Kotz will oversee the administration and usage of the equipment under the full support of Minneapolis VAHCS Director, Mr. Patrick J. Kelly and the Research & Development Committee Chair, Dr. Ronald Bach. The major and minor user groups for this equipment span multiple VA research disciplines and represents both basic and translational research in areas such as: aging, cardiovascular, cancer, neuroscience, stem cell therapies and obesity. The VA funded users combined will be used at least 75% of the instrument capacity. The minor users group will occupy between 25%-30% of instrument capacity. The ability for Minneapolis VAHCS to continue to remain one of the largest and most active research programs in the VAHCS depends on the ability of its investigators to gain access to the newest generation of high-sensitivity, high-resolution instruments. The acquisition of the Promethion continuous cage metabolic phenotyping systems requested would accelerate and enhance the rate at which researchers from multiple disciplines could acquire, analyze, display, and understand data with the long-term goal of benefiting the unique health care needs of US Veterans. This not only benefits the progress of VAHCS research in general but it would benefit the health care of all Americans.