Enrichment Program

摘要（丰富计划） 光学成像技术和数字图像分析的非凡发展开辟了新的 有机会研究离体标本以及活体动物的生理学和病理生理学。 多光子显微镜、FLIM和定量3D细胞仪等技术将我们的探测范围扩展到 包括高分辨率亚细胞细节和大规模定量体积分析。传播 这些技术及其在肾脏和泌尿外科研究中的应用有可能提供重要的 这是其他方法无法获得的新见解。因此，强化计划服务于 奥布莱恩中心和国家和国际社会的研究人员在肾脏和泌尿系统疾病， 将中心开发的技术和资源提供给尽可能广泛的 investigators.核心的三个具体活动将实现这一目标：1）年教学研讨会 1、3和5将肾脏和泌尿外科领域的研究人员与光学领域的主要权威人士聚集在一起， 成像，以获得在一个论坛，促进先进的光学显微镜方法的实践培训 对话和解决问题; 2）访问演讲者计划，使成像领导人与中心互动 调查人员和工作人员以及当地研究基地; 3）开发一个全面的网络资源 传播方法、教材和软件。核心的这些活动将 补充和扩大该中心其他核心的其他非正式外联活动。的 因此，充实方案是中心的一个重要组成部分，有助于中心为 国家和国际肾脏和泌尿系统疾病研究者协会。

Abstract (Enrichment Program) Extraordinary developments in optical imaging technologies and digital image analysis have opened new opportunities to study physiology and pathophysiology in ex vivo specimens as well as the live animal. Techniques such as multiphoton microscopy, FLIM and quantitative 3D cytometry extend our probing reach to encompass both high resolution subcellular details and large-scale quantitative volume analysis. Dissemination of these technologies and their application in renal and urologic research has the potential to provide important new insight that cannot be obtained using other approaches. Therefore, the Enrichment Program serves the O'Brien Center and the national and international community of researchers in renal and urologic diseases by making available the technologies and resources developed in the Center to the widest possible range of investigators. Three specific activities of the Core will accomplish this goal: 1) instructional workshops in years 1, 3 and 5 to bring investigators in the renal and urologic field together with the leading authorities in optical imaging to obtain hands-on training in advanced methods of optical microscopy in a forum that facilitates dialogue and problem solving; 2) a visiting speakers program to bring leaders in imaging to interact with Center investigators and staff and with the local research base; and 3) development of a comprehensive web resource for dissemination of methods, educational materials and software. These activities of the Core will complement and extend other informal outreach activities of the other cores comprising this Center. The Enrichment Program, therefore, is an essential component of the Center that facilitates its service to the national and international community of investigators in renal and urologic diseases.