C-Trap optical tweezers

项目摘要/摘要 我们正在请求资金购买LUMICKS C-Trap光学镊子仪器，这是一个集成的 结合三色的单分子生物化学和生物物理研究解决方案 荧光显微镜、光学镊子和具有定制用户界面的五通道微流体 仪器控制和自动化。C-Trap的功能非常广泛，可以进行实时观察 蛋白质-蛋白质和蛋白质-核酸相互作用的分析，液-液相分离，蛋白质 折叠和构象变化，细胞力学和反应，以及细胞结构和运输。这个 预期的用户基础同样广泛，包括染色体生物学家、分子遗传学家、结构 病毒学家和化学生物学家。进入C-Trap将迅速促进单分子实验 包括对DNA修复、转录调控、蛋白质等机制的基础研究 贩运和质量控制，DNA复制，水平基因转移，亚细胞定位 生物分子和蛋白质的小分子靶向。从长远来看，使用C-Trap收集的数据将 帮助一大批由NIH支持的研究人员在了解基础癌症生物学方面取得进展，宿主- 病原体相互作用，抗生素耐药性，蛋白质(非)折叠疾病，以及一系列发育和 与正在研究的蛋白质家族相关的神经病理学。我们进一步预计，C-Trap可以 用于在印第安纳大学和邻近机构培养单分子研究人员社区， 以及培养下一代单分子实验者。总体而言，购买C-Trap 将导致在顶级期刊上发表有影响力的文章，使用户能够更好地竞争外部资金 并在他们的研究和人类健康领域产生积极影响。

PROJECT SUMMARY/ABSTRACT We are requesting funds to purchase a LUMICKS C-Trap optical tweezers instrument, which is an integrated solution for single-molecule biochemistry and biophysics investigations that incorporates three-color fluorescence microscopy, optical tweezers, and five-channel microfluidics with a custom user interface for instrument control and automation. The capabilities of the C-Trap are broad, enabling the real-time observation and analysis of protein-protein and protein-nucleic acid interactions, liquid-liquid phase separation, protein folding and conformational changes, cell mechanics and response, and cellular structure and transport. The anticipated user base is equally broad, including chromosome biologists, molecular geneticists, structural virologists, and chemical biologists. Access to the C-Trap will quickly facilitate single-molecule experiments involving fundamental investigations into the mechanisms of DNA repair, transcriptional regulation, protein trafficking and quality control, DNA replication, horizontal gene transfer, the subcellular localization of biomolecules, and small-molecule targeting of proteins. In the long term, the data gathered using the C-Trap will help a large group of NIH-supported researchers to make progress in understanding basic cancer biology, host- pathogen interactions, antibiotic resistance, protein (un)folding diseases, and an array of developmental and neurological pathologies related to the protein families being studied. We further anticipate that the C-Trap can be used to foster a community of single-molecule researchers at Indiana University and neighboring institutions, as well as to train the next generation of single-molecule experimentalists. Overall, the purchase of the C-Trap will lead to high-impact publication in top tier journals, enabling users to better compete for extramural funding and make a positive impact in their fields of research and human health.