Recruitment, Data Collection & Sample Management Core

梁摘要：核心B 儿童哮喘的患病率在生活在不同环境中的人群之间差异很大， 这表明，产前和生命早期的环境暴露在确定这种常见的风险方面发挥了作用。 通过影响肠道微生物群和免疫发育模式来预防疾病。两国早期哮喘 和微生物组研究（BEAMS）计划项目利用了我们自己后院的自然实验 阐明哮喘保护的微生物来源。它将在两个人群中开展一项出生队列研究， 共同的祖先，但显着不同的环境，我们已经表明，有高和低的患病率 在亚利桑那州图森市出生的墨西哥裔美国儿童和在墨西哥诺加莱斯出生的儿童， 分别招募、数据收集和样本管理核心（核心B）对此至关重要 因为其将指导、协调和完成BEAMS入组受试者的所有数据收集。核心 B将为本申请中提出的所有三个项目提供服务，为项目1和所有 项目2和项目3中使用的微生物和环境样本。核心课程B的具体目标是：1）招收250人 亚利桑那州图森市的墨西哥裔美国妇女和墨西哥诺加莱斯的250名墨西哥妇女在妊娠晚期 获取免疫学和微生物组评估样本，获取环境样本， 和管理问卷，以确定产前暴露和潜在的协变量; 2）遵循 这些妇女的孩子从出生到生命的前24个月，反复采集粪便和血液样本， 获取环境样本，并对接触和并发疾病进行问卷调查， 可能影响哮喘风险或肠道微生物组的发展和临床结果;以及3）提供 收集BEAMS受试者所有数据的资源，并管理获得的所有样本和问卷 从现场采集到实验室样品处理，包括库存控制、数据录入和 质量控制和分发到适当的实验室。核心B将提供收集资源 BEAMS受试者的所有数据，包括有受试者招募经验的人员、家庭环境 评估、血液采样和问卷管理，一个实验室，用于清点和处理 环境样本，以及过境运输标本的基础设施。核心B将利用 与两个综合性健康中心建立了持久的关系，这两个中心将成为我们的临床合作伙伴， 招募和随访母婴二对体。核心B团队包括国家领导人， 非常成功的出生队列研究和边境卫生，他们与社区建立了长期关系 将参与这项研究。通过对样本和数据收集采取协调一致的办法， B将确保获得有关孕妇及其出生子女的全面信息 在这一独特的边境环境中，我们将在24个月内完成这一项目，从而确保该项目将实现其总体目标。

BEAMS ABSTRACT: Core B The prevalence of childhood asthma varies dramatically between populations living in different environments, suggesting that pre-natal and early life environmental exposures play a role in establishing risk for this common disease through influencing patterns of gut microbiota and immune development. The Binational Early Asthma and Microbiome Study (BEAMS) Program Project takes advantage of a natural experiment in our own backyard to elucidate the microbial origins of asthma protection. It will initiate a birth cohort study in two populations with shared ancestry but dramatically different environments, which we have shown to have high and low prevalence of asthma: Mexican American children born in Tucson, Arizona and children born in Nogales, Mexico, respectively. The Recruitment, Data Collection and Sample Management Core (Core B), is critical to this effort as it will direct, coordinate and complete all data collection on subjects enrolled in BEAMS. Core B will serve all three projects proposed in this application, collecting blood and data for Project 1 and all the microbiota and environmental samples used in Projects 2 and 3. Specific aims of Core B are to: 1) Enroll 250 Mexican American women in Tucson, Arizona and 250 Mexican women in Nogales, Mexico in the third trimester of pregnancy, obtain samples for immunologic and microbiome assessments, obtain environmental samples, and administer questionnaires for identification of prenatal exposures and potential covariates; 2) Follow the children of these women from birth through the first 24 months of life, sampling stool and blood repeatedly, obtaining environmental samples and administering questionnaires on exposures and intercurrent illnesses that may influence asthma risk or the development of the gut microbiome and clinical outcomes; and 3) Provide the resources for collection of all data on BEAMS subjects and manage all samples and questionnaires obtained from collection in the field through sample processing in the labs, including inventory control, data entry and quality control, and distribution to the appropriate laboratories. Core B will provide the resources for collection of all data on BEAMS subjects, including personnel experienced in subject recruitment, home environmental assessments, blood sampling and questionnaire administration, a laboratory for inventorying and processing of environmental samples, and an infrastructure for specimen transport across the border. Core B will leverage enduring relationships with two comprehensive health centers that will serve as our clinical partners for recruitment and follow-up of mother/infant dyads. The Core B team includes national leaders in the conduct of highly successful birth cohort studies and border health who bring long-term relationships with the communities that will participate in the study. By implementing a coordinated approach to sample and data collection, Core B will assure that comprehensive information is obtained on pregnant women and their children from birth through 24 months in this unique border setting, thereby assuring the Program will achieve its overarching goals.