Facility Core D: Biomarkers

毒理基因组学和蛋白质组学工具使研究人员能够快速筛选大细胞中的表达变化， 许多基因和蛋白质对有毒物质的暴露作出反应，从而能够有效地识别 暴露、生物效应和/或易感性的潜在分子生物标志物。但调查人员 传统上，他们的研究集中在特定分子途径中预先存在的假设上。 许多研究人员缺乏试剂、时间、生物信息学工具和技术专长来存储、管理、 分析、确认和跟踪通过毒理基因组学和蛋白质组学分析观察到的令人兴奋的变化， 生物学途径是他们的先验假设或专业领域之外。的主要作用 达特茅斯超级基金基础研究计划（SBRP）的一个新的生物标志物核心（核心D）将协助 SBRP研究人员使用这些毒理基因组学和蛋白质组学工具来识别潜在的分子 暴露、生物效应和/或易感性的生物标志物。我们将协助该计划的研究人员， 利用核心A（分子生物学和蛋白质组学核心）以及达特茅斯微阵列， 生物信息学共享资源为他们的个人项目。目标将是协助调查人员 这SBRP计划在实现其科学项目的具体目标，在最有效的， 在毒理基因组学、毒理蛋白质组学和生物标志物开发领域的生产方式， 应用程序.核心小组还将协助开发、验证和应用新的方法 可以用来评估暴露的功能效应。这些生物标志物可以应用于基础 机制研究或在分子流行病学和生态毒理学领域的研究， 与接触砷和其他有毒金属有关的影响。

Toxicogenomic and proteomic tools allow investigators to rapidly screen for expression changes in large numbers of genes and proteins in response to toxicant exposures that enable efficient identification of potential molecular biomarkers of exposure, biological effect, and/or susceptibility. However, investigators have traditionally focused their research on pre-existing hypotheses within a specific molecular pathway. Many researchers lack the reagents, time, bioinformatics tools, and technical expertise to store, manage, analyze, confirm and follow-up on exciting changes observed through toxicogenomic and proteomic assays in biological pathways that are outside of their a priori hypotheses or specialty areas. The principal role of the new Biomarkers Core (Core D) of the Dartmouth Superfund Basic Research Program (SBRP) will be to assist SBRP researchers in using these toxicogenomic and proteomic tools to identify potential molecular biomarkers of exposure, biological effect, and/or susceptibility. We will assist the program's researchers in utilizing Core A (Molecular Biology & Proteomics Core) as well as the Dartmouth Microarray and Bioinformatics Shared Resources for their individual projects. The goal will be to assist the investigators of this SBRP program in accomplishing the specific aims of their scientific projects in the most efficient and productive manner in the area of toxicogenomics, toxicoproteomics and biomarkers development and application. The Core will also assist in the development, validation and application of new methodologies that can be used to assess the functional effects of exposures. These biomarkers can then be applied in basic mechanistic studies or in molecular epidemiology and ecotoxicology field studies examining the adverse effects associated with exposure to arsenic and other toxic metals.