ANIMAL CORE

MARC动物核心将为中心提供采购，测试，繁殖，维护和分发服务 动物研究者的研究动物核心将服务于五个主要目的：（1）购买和建立 饲养员为MARC Scientific Components生产动物;（2）相关的行为测试小鼠 随着科学组件5和6使用的短期选择线的创建（3）确定 动物分配的优先顺序和制定分配程序;（4）开发新的行为测试 程序，并根据需要获得动物，用于新的试点项目或方向的变化， （5）维护一个数据库，用于跟踪个体动物并为所有MARC研究者提供性状数据 查看和使用。C57 BL/6 J（B6）和DBA/2 J（D2）近交系的后代F1或F2小鼠 小鼠将用于科学组件5和6以及试点项目8B。核心将培育F1和F2 用于这些组件的鼠标。选择一组重复的小鼠品系用于高和低 甲基苯丙胺（MA）饮用，并为高和低MA诱导的致敏性的独立集将 在Animal Core内创建，用于分发给科学组件5和6。B6育种者也将被设置 最多生产出在试点项目8 C核心区接受治疗的新生儿。动物中心将由 约翰·克拉布博士，他在开发成瘾动物模型方面经验丰富， 根据需要为新飞行员提供其他品系或基因型的小鼠。所有动物均将圈养 在AALAC认证的动物设施中，在有执照的兽医的全职监督下进行。程序 为避免病毒和细菌污染，并使用哨兵筛查计划 以快速识别污染并避免扩散。仔细的基因记录和鉴定 保持大量基因型的实践已经存在了几年。关键 Animal Core的优势是与集中开发、行为测试、 选择系小鼠的分布;控制环境条件;遗传质量控制; 从组件Pis中去除动物生产负担。关于动物分布的决定将是 集中管理，并基于一系列标准，包括请求日期、请求是否为机读目录 项目、科学进展和最后分发给该项目的日期。核心将能够分发 跨项目同窝出生，以尽量减少研究中的窝影响，并增加交叉实验的可能性 关联分析核心做法将加强机读目录产生的数据。

The MARC Animal Core will serve the Center by purchasing, testing, breeding, maintaining and distributing animals to MARC investigators. The Animal Core will serve five major purposes: (1) Purchase and set up breeders to produce animals for the MARC Scientific Components; (2) Behaviorally test mice associated with the creation of short-term selection lines to be used by Scientific Components 5 and 6 (3) Determine animal distribution priorities and develop distribution procedures; (4) Develop new behavioral testing procedures and obtain animals on an as needed basis for new pilot projects or changes in direction as they occur; (5) Maintain a database that will track individual animals and post trait data for all MARC investigators to view and utilize. F1 or F2 mice that are the descendents of C57BL/6J (B6) and DBA/2J (D2) inbred strain mice will be used in Scientific Components 5 and 6, and Pilot Project 8B. The Core will breed F1 and F2 mice for use in these components. A set of replicated mouse lines selected for high and low methamphetamine (MA) drinking, and an independent set for high and low MA-induced sensitization will be created within the Animal Core for distribution to Scientific Components 5 and 6. B6 breeders will also be set up to produce the neonates to be treated in the Core for Pilot Project 8C. The Animal Core will be managed by Dr. John Crabbe, who is experienced in developing animal models of addiction, and will be capable of providing other strains or genotypes of mice for new pilots on an as needed basis. All animals will be housed in AALAC accredited animal facilities under the full-time supervision of a licensed veterinarian. Procedures are in place for the avoidance of viral and bacterial contamination, and a sentinel screening program is used to quickly identify contamination and avoid spread. Careful genetic record-keeping and identification practices for the maintenance of a large number of genotypes have been in place for several years. Key advantages of the Animal Core are cost savings associated with centralized development, behavioral testing and distribution of the selected line mice; controlled environmental conditions; genetic quality control; removal of animal production burden from Component Pis. Decisions about animal distribution will be centralized and based on a number of criteria, including date of request, whether the request is for a MARC project, scientific progress, and date of last distribution to that project. The Core will be able to distribute littermates across projects to minimize litter effects within studies, and increase the potential for crossexperiment association analyses. Core practices will strengthen the data generated by the MARC.