Equipment Fund

预算合理性--协作发展基金 我们要求每年提供135,000美元，以建立协作发展基金。广泛的合作者 将被征募以帮助进一步鉴定和验证HIV-宿主蛋白复合体，促进结构 络合物的测定，利用结构信息更深入地探索其生物学功能 HIV调节蛋白和辅助蛋白，并在高通量方面扩展技术开发 表达、结构测定、MS方法、冷冻-EM和断层扫描。我们将以一种方式 促进基本的合作将是为有前途的研究提供种子资金。这些研究可能 包括试剂制备、诱变、生化、功能或病毒学实验、方法 识别和验证新的复合体，或其他方法开发。请拨的资金将提供 灵活地参与新的方向，并将主要用于资助与外部调查人员的分包合同， 这是与我们的咨询委员会协商并经NIGMS批准后确定的。这一类别的基金 还将分配给现有和潜在合作者对HARC中心的访问，以规划未来的研究 方向。

BUDGET JUSTIFICATION - Collaboration Development Fund We request $135,000 per year to establish a Collaboration Development Fund. A wide range of collaborators will be enlisted to help further identify and validate HIV-host protein complexes, facilitate structure determination of the complexes, utilize structural information to more deeply probe the biological functions of the HIV regulatory and accessory proteins, and extend technological developments in high-throughput expression, structure determination, MS methods, cryo-EM, and tomography. One way in which we will facilitate essential collaborations will be to provide seed funding for promising studies. These studies may include reagent preparation, mutagenesis, biochemical, functional, or virological experiments, methods to identify and validate new complexes, or other methods development. The requested Fund will provide flexibility to engage in new directions and will be used primarily to fund sub-contracts with outside investigators, as determined in consultation with our advisory committees and with NIGMS approval. Funds in this category also will be allocated to visits of existing and potential collaborators to the HARC Center to plan future research directions.