Core D: Neuropathology and Animal Behavior

神经病理学和动物行为核心的使命将是提供 NIEHS中心也有来自人类帕金森病病例的具有良好特征的尸检脑材料 与表达α-突触核蛋白、DJ-1、Parkin和PINK1的转基因动物模型一样 用于神经病理、分子、生化和行为研究。核心将提供 从帕金森病患者的人体组织和帕金森病动物模型到项目1、2和3的支持； 支持项目3中的行为研究；并帮助进行生存/凋亡/坏死分析 项目4的人类胚胎干细胞(HESC)来源的发育中的神经元。 发展最先进的神经退行性变的神经病理分析技术， 错误折叠蛋白堆积的生化分析，以及运动和运动的行为分析 认知改变。此外，我们还开发了独特的帕金森病动物模型，以便在其中进行测试 并筛选新的分子途径和小分子。请注意，人类诊所 NIEHS Center RFA中定义的组件包含在此核心中。

The mission of the Neuropathology and Animal Behavior Core will be to provide the projects of the NIEHS Center with well-characterized autopsy brain material from human PD cases as well as with transgenic (tg) animal models expressing a-synuclein (a-syn), DJ-1, Parkin and PINK1 for neuropathological, molecular, biochemical and behavioral studies. The Core will provide support with human tissues from PD patients and animal models of PD to Projects 1, 2, and 3; support behavioral studies in Project 3; and help with survival/apoptosis/necrosis analysis of human embryonic stem cell (hESC)-derived developing neurons for Project 4. The Core has developed state of the art techniques for neuropathological analysis of neurodegeneration, biochemical analysis of misfolded protein accumulation, and behavioral analysis of motor and cognitive alterations. Moreover, we have developed unique animal models of PD in which to test and screen new molecular pathways and small molecules. Please note that a human clinical component, as defined in the NIEHS Center RFA, is contained within this Core.