Structure Biology Core

请参阅说明)： 纽约大学医学院孔祥鹏博士的团队将作为结构生物学核心(SBC) 项目名称为“针对HIV-1ENV的CD4结合区的中和抗体” 对NIAID项目公告PAR-06-285，艾滋病毒疫苗研究和设计(HIVRAD)的回应。这个 P01的团队除了孔博士的团队外，还包括山路博士(Pi，马萨诸塞大学 ；Paul Clapham(马萨诸塞大学医学院)；和Shau-look Hu(大学 华盛顿)。我们的SBC团队在蛋白质结晶学、冷冻电子显微镜方面经验丰富 和其他生物物理技术，我们将在三个方面为P01团队提供服务：(I)使用蛋白质 用结晶学方法鉴定模拟HIV-1 CD4结合位点的重组蛋白 Gp120，以及P01团队产生的单抗的结构特征； 表面等离子体共振(SPR)方法研究HIV-1gp120与其相互作用 具有其受体CD4以及针对HIV-1表面蛋白的抗体的突变体；(Iii)使用计算 方法表征gp120及其突变体的结构动力学。结构信息 由我们的SBC产生的数据将有助于POTS旨在设计一种针对HIV-1的疫苗。 相关性(请参阅说明)：

Seeinstructions): Dr. Xiangpeng Kong's group at NYU School of Medicine will serve as the Structural Biology Core (SBC) for a Program Project entitled "Neutralizing antibodies targeting the CD4 binding region of HIV-1 ENV" in response to NIAID program announcement PAR-06-285, HIVVaccine Research and Design (HIVRAD). The team of this P01 includes, in addition to Dr. Kong's group, Drs. Shan Lu (PI, University of Massachusetts Medical School); Paul Clapham (University of Massachusetts Medical School); and Shiu-Lok Hu (University of Washington). Our SBC team is highly experienced in protein crystallography, cryo-electron microscopy and other biophysical techniques, and we will provide services for the P01 team in 3 areas: (i) using protein crystallographic methods to characterize a recombinant protein that mimics the CD4 binding site of HIV-1 gp120, and structural characterization of monoclonal antibodies generated by the P01 team; (ii)using surface plasma resonance (SPR) method to characterize the interactions between HIV-1 gp120 and its mutants with its receptor CD4 as well as antibodies against the HIV-1 surface protein; (iii)using computation method to characterize the structural dynamics of gp120 and its mutants. The structural information generated from our SBC will help the POTs aim in designing a vaccine against HIV-1. RELEVANCE (Seeinstructions):