Stem Cell and Xenografts

核心C将支持和鼓励皮肤上皮干细胞的研究，提供专业知识和 毛囊上皮干细胞的鉴定和分离方面的培训。CORE的主要目标 C是使研究人员能够分析他们的转基因或突变小鼠上皮干细胞的变化 并鼓励使用人皮肤/SCID小鼠异种移植模型将小鼠的工作转化为人皮肤。 CORE C也利用了在CORE主任实验室中发现的干细胞的分析和专业知识 作为在宾夕法尼亚大学建立的现有资源和成功的核心。特别是，Core C将与 宾夕法尼亚大学医学院流式细胞术核心流出病理科与异种移植 医学部的核心部分由再生医学研究所提供资金。 这些互动将确保调查人员能够获得高质量、高成本效益的服务，因为 以及在他们的研究工作中研究上皮干细胞所需的科学专业知识。 干细胞核心将提供对毛囊干细胞和前体细胞的分析，使用各种标准 (标记保留细胞、免疫组织化学)和前沿分析(荧光标记分析 激活的细胞分选[FACS]，多能试验)。Core C将提供毛囊干细胞的功能测试 和祖细胞在重建实验中的表达。核心的主要部分将是为实验室提供培训 人员，以便他们可以从皮肤中分离细胞群，并执行FACS分析和细胞 使用已建立的流式细胞术核心进行分离。 Core C的人类皮肤异种移植部分将鼓励测试小鼠的研究结果与人类的相关性 皮肤。异种移植模型是在人体皮肤上进行临床前功能研究的一种优雅手段 将简化临床相关技术的开发。 核心C将通过使用核心A的免疫组织化学服务来评估干细胞，从而与核心A整合 组织中的标志物。核心C将通过接受异种移植的皮肤与核心B整合。

Core C will support and encourage research into cutaneous epithelial stem cells by providing expertise and training in the characterization and isolation of epithelial stem cells in the hair follicle. The main goals of Core C are to enable investigators to analyze their transgenic or mutant mice for alterations in epithelial stem cells and to encourage translation of mouse work to human skin using a human skin/scid mouse xenograft model. Core C takes advantage of assays and expertise in stem cells found in the core director's laboratory, as well as existing resources and successful cores established at Penn. In particular, Core C will integrate with Penn's School of Medicine Flow Cytometry Core run out of the pathology department and the Xenografting core in the department of medicine that is partly funded through the Institute for Regenerative Medicine. These interactions will assure that investigators will have access to high quality, cost effective services, as well as the scientific expertise necessary to study epithelial stem cells in their research efforts. The stem cell core will offer analysis of hair follicle stem and progenitor cells using a wide variety of standard (label-retaining cell, immunohistochemistry) and cutting edge assays (marker analysis by fluorescent activated cell sorting [FACS],.multipotency testing). Core C will offer functional testing of hair follicle stem and progenitor cells in reconstitution assays. A major part of the core will be to provide training to laboratory personnel so that they can isolate cell populations from the skin and perform FACS analysis and cell isolation using the established Flow Cytometry Core. The human skin xenograft portion of Core C will encourage testing relevance of mouse findings in human skin. The xenograft model is an elegant means to perform preclinical functional studies on human skin that will streamline the development of clinically relevant technologies. Core C will integrate with Core A by using Core A's immunohistochemistry services to assess stem cell markers in tissues. Core C will integrate with Core B by receiving skin for xenografting.