Identification of Altered Glycan and Glycoproteins in Viral Induced Liver Cancer

这项研究拨款将试图确定所有血清糖蛋白水平升高的 肝细胞癌患者血清中岩藻糖基化的研究这在文献中是公认的 糖基化的变化会在癌症中发生。更重要的是，我们已经表明， 核心岩藻糖化水平与肝癌的发生有关。然而，其上的蛋白质 这些变化发生在哪些地方，在很大程度上是未知的。因此，我们建议在两种情况下评估血清 癌前状态和癌状态，与所有血清相关的岩藻糖化量的变化 糖蛋白。这项比较研究将使用一种将利用各种岩藻糖识别的技术 凝集素用于提取特定的糖蛋白，并使用基于LC MS/MS的蛋白质组学方法进行鉴定。 随着癌症的发展而改变的糖蛋白将被进一步评估其潜在的 病毒诱发肝癌的生物标志物。

This research grant will attempt to identify all the serum glycoproteins that have increased levels of fucosylation in the sera of patients with hepatocellular carcinoma (HCC). It is acknowledged in the literature that changes in glycosylation occur with cancer. More importantly we have shown that there is an increase in the level of core fucosylation associated with the development of liver cancer. However, the proteins on which these changes occur are, for the most part, unknown. Hence, we propose to evaluate serum, in both a pre cancerous and cancerous state, for alterations in the amount of fucosylation associated with all serum glycoproteins. This comparative study will use a technology that will exploit a variety of fucose recognizing lectins to extract specific glycoproteins and identify them using LC MS/MS based proteomic methodologies. Glycoproteins that alter with the development of cancer will be evaluated further for their potential as biomarkers of virally induced liver cancer.