Biocontainment Research Support Services Core

项目概要：RMRBL UC 7 Core 3 落基山地区生物防护实验室（RMRBL）的构想是伴随着 生物防御/新兴传染病卓越研究中心。的 这些基础设施和研究方面的协同投资的结果是一个多学科的 研究团队和核心研究服务。RMRBL应对新兴风险的能力 和持续的病原体威胁是由于我们对病原体的集体理解 感染的特点、致病机理、免疫功能和宿主范围。我们的快速生产历史 应对对公共和社会经济健康构成威胁的高后果病原体， 这不仅是由于驻地调查员小组的各种力量，而且也由于我们有能力 在BSL 3设施中进行这些重要研究。具体而言，CSU RMRBL在以下方面具有优势 确定和优化病原体和病原体灭活的体外培养条件， 建立必要的动物模型，以调查野生动物和家畜的溢出 事件和水库潜力，以及使用活的和灭活的感染性 体外生产的材料。此外，CSU RMRBL拥有尖端技术的专业知识 研究微生物生理学和动物模型;如进行蛋白质组学和 代谢组学质谱分析，使用空间分析的详细组织病理学分析， 转录组学和AI驱动的定量病理学评分。最后，我们的集体研究团队 已经建立了提供生物试剂的计划，这些生物试剂可以稳健地用于动物， 和人类临床研究，包括灭活的生物材料， 在BSL 3之外可重复使用。在这个核心中，我们将我们的集体力量集中在一起， RMRBL的BSL 3设施内的生物防护研究资源核心。将该得到的 病原体表征核心设计我们的能力，通过独立和高度互动 目标1：大流行病病原体的自然传染源和外溢威胁 目标2：用于疫苗和诊断研究的试剂和模型， 目标3：病原体：处理宿主材料以确定分子量 光谱成像和单细胞组学分析。 RMRBL BSL 3病原体表征核心提供了协同活性的机会， 扩大我们在RBL网络和其他合作伙伴中的资源，打造新技术 在RMRBL BSL 3中，并最终提高我们适应和快速交付研究的能力 我们需要对威胁我们卫生系统的病原体作出敏锐反应的资源。

Project Summary: RMRBL UC7 Core 3 The Rocky Mountain Regional Biocontainment Laboratory (RMRBL) was conceived concomitant with a Research Center for Excellence in Biodefense / Emerging Infectious Diseases. The outcome of these synergized investments in infrastructure and research was a multidisciplinary team of investigators and core research services. The RMRBL’s ability to respond to emerging and persistent pathogen threats are due to our collective understanding of pathogen characteristics, pathogenesis of infection, immunity and host range. Our prolific history of rapid response to high consequence pathogens posing a threat to public and socioeconomic health is due not only to the diverse strengths of the resident investigator teams, but also our ability to conduct these important studies in BSL3 facilities. Specifically, the CSU RMRBL has strengths in defining and optimizing in vitro culture conditions for pathogens and pathogen inactivation, establishing the requisite animal models to investigate wildlife and domestic animal spillover events and reservoir potential, and vaccine models using both viable and inactivated infectious materials produced in vitro. Further, the CSU RMRBL has expertise in cutting-edge technologies to study microbial physiology and animal models; such as conducting proteomic and metabolomics mass spectrometry analyses, detailed histopathologic analyses using spatial transcriptomics and AI-driven quantitative pathology scoring. Lastly, our collective research teams have established programs for supplying biological reagents that can be robustly used for animal and human clinical research, including inactivated biomaterials that can be safely and reproducibly used outside the BSL3. In this Core, we culminate our collective strengths into one Biocontainment Research Resources Core within the BSL3 facilities of the RMRBL. This resultant pathogen characterization core devises our capacity through independent and highly interactive aims; Aim 1: Natural reservoirs of infection and spillover threat for pathogens of pandemic potential; Aim 2: Reagents and models for vaccine and diagnostics studies for pathogens of pandemic potential; and Aim 3: Pathogen:Host materials processing for molecular mass spectrometry imaging and single cell ‘omics analyses. The RMRBL BSL3 Pathogen Characterization core provides opportunities to synergize activities, expand our resources across the RBL network and to additional partners, forge new technologies within the RMRBL BSL3, and ultimately improve our ability to adapt and rapidly deliver research resources acutely responsive to pathogens possessing a threat to our health systems.