Administrative Supplement for Equipment Purchase

项目摘要 核RNA的加工和监视是一个复杂的过程，涉及多种蛋白质和蛋白质 这些组件识别并最终降解靶向RNA底物。RNA解旋酶Mtr 4 在这一过程中的核心作用，通过提供底物靶向和RNA衰变之间的桥梁， RNA外泌体。RNA识别直接由Mtr 4和各种衔接蛋白进行， Mtr 4至靶向底物。一个主要的挑战是了解这些分子的细节， 蛋白质与RNA底物相互作用并相互作用以实现底物特异性。此外，本发明还 Mtr 4内的辅助结构域在底物识别中起重要但不明确的作用， 处理.值得注意的是，可用的结构数据表明Mtr 4是一种高度动态的蛋白质，但它 蛋白质动力学如何与解旋酶功能相关尚不清楚。该项目旨在定义分子 通过采用各种生物化学、生物物理学和结构方法， 通过体内遗传分析得到支持。本项目的具体目标是：（1）描述 Mtr 4附属结构域和（2）表征TRAMP（Trf 4-Air2-Mtr 4）复合物内的相互作用。 对生物医学研究的未来职业感兴趣的本科生和研究生研究人员，或 医学将参与该项目。

Project Summary Nuclear RNA processing and surveillance is a complex process involving multiple proteins and protein assemblies that identify and eventually degrade targeted RNA substrates. The RNA helicase, Mtr4, plays a central role in this process by providing a bridge between substrate targeting and RNA decay by the RNA exosome. RNA recognition is performed directly by Mtr4 and by various adaptor proteins that recruit Mtr4 to targeted substrates. A major challenge is to understand the molecular details of how these proteins interact with RNA substrates and with each other to achieve substrate specificity. Additionally, accessory domains within Mtr4 play important but poorly defined roles in substrate recognition and processing. Notably, the available structural data demonstrate that Mtr4 is a highly dynamic protein, but it is not known how protein dynamics relate to helicase function. This project seeks to define the molecular basis for Mtr4 function by employing a variety of biochemical, biophysical and structural approaches, supported by in vivo genetic analyses. The specific aims of this project are to (1) describe the role of Mtr4 accessory domains and (2) characterize interactions within the TRAMP (Trf4-Air2-Mtr4) complex. Undergraduate and graduate researchers with interests in future careers in biomedical research or medicine will be involved in the project.