Viral vector technology for cell type specific gene delivery

项目摘要 此行政补充是购买索尼台式SH 800细胞分选机。细胞分选对于 我们合理设计腺相关病毒嗜性的方法（参见R 01 GM 141152“病毒载体技术 用于细胞类型特异性基因递送”）。在明尼苏达大学的Flow Core设施中，细胞分选仅可用于 作为由流程核心工作人员执行的“收费服务”。在这种限制下，我们遇到了两个问题：1） 由于Flow Core的两台分拣机的使用率很高，我们很难确保有足够的时间进行 在我们的R 01中概述的研究。2)对于许多试验/优化实验，Flow Core的高吞吐量 分类器没有得到充分利用，它们的复杂性实际上是一个障碍。台式分拣机将缩短时间 连续的AAV设计/测量/优化周期所需的时间，从而能够加速AAV设计/测量/优化周期的时间轴。 在我们的R 01中概述的实验。综上所述，需要购买一个专门用于该项目的细胞分选机， 紧急

Project Summary This administrative supplement is to purchase a Sony benchtop SH800 cell sorter. Cell sorting is essential for our approach to rationally engineer Adeno-Associated Virus tropism (see R01GM141152 ”Viral vector technology for cell type specific gene delivery”). At the University of Minnesota Flow Core facility, cell sorting is only available as a ‘fee-for-service’ performed by a flow core staff member. With this limitation, we have run into two issues: 1) Because of heavy utilization of the Flow Core’s two sorters, we have trouble securing enough time to conduct research outlined in our R01. 2) For many pilot/optimization experiments, the high throughput of the Flow Core’s sorters is underutilized, and their complexity is actually a hindrance. A benchtop sorter will shorten the time required for consecutive AAV design/measure/optimize cycles and thus enable an accelerated timeline of experiment outlined in our R01. Taken together, the need to purchase a cell sorter dedicated to this project is urgent.