Role of Redox Dependent Signaling in Leukemia

描述（由申请人提供）：CML（慢性髓性白血病）的独特之处在于BCR/ABL癌基因驱动疾病的发生和进展。抑制BCR/ABL激酶的疗法，如甲酸伊马替尼，已经显著地改变了慢性疾病的治疗，但在原细胞危象和BCR/ABL的特定点突变中效果较差。Blast危机是该病的一个非常具有侵袭性的阶段，其特征是基因组不稳定和继发性突变。这些特征与活性氧（ROS）引起的DNA损伤有关。我们和其他人发现p210 BCR/ABL的过表达会导致ROS水平升高。我们的初步数据显示，ROS水平升高的结果是Fyn （Src激酶家族成员）的上调。我们假设通过Fyn转导的ROS依赖信号有助于细胞危象的进展。BCR/ABL过表达细胞Fyn蛋白表达上调4倍。这种上调被抗氧化剂阻断，从而将ROS水平的增加与Fyn表达联系起来。BCR/ABL中赋予甲磺酸伊马替尼耐药性的点突变保留了Fyn的上调，这表明Fyn可能是难治性患者的合适治疗靶点。使用shRNA敲低Fyn可减缓白血病细胞体外和体内生长，抑制克隆生长45%，并导致对伊马替尼的敏感性增加。这些显著的变化表明Fyn对白血病细胞增殖和对伊马替尼的敏感性很重要。阻断Fyn也会降低细胞内过氧化物的总体水平，这表明Fyn可以放大ROS水平，从而导致随后的DNA损伤和继发性突变。这些初步数据将Fyn置于ROS增加的下游和上游，表明存在正反馈回路，Fyn通过BCR/ABL激活导致ROS增加，可能在爆炸危机中发挥重要作用。这一建议将阐明氧化依赖性Fyn上调的机制和后果，并在小鼠模型和临床标本中验证这些发现。本提案的具体目的是：#1：确定BCR/ABL和ROS水平上调Fyn的机制。# 2：评估Fyn上调对ROS依赖性增殖信号和DNA损伤的影响。#3：在CML小鼠模型和患者标本中测试BCR/ABL启动的ROS和Fyn上调对CML进展的相对贡献。这些数据将深入了解BCR/ abl介导的ROS改变的一个重要后果：Fyn上调。我们的长期目标是了解白血病中氧化依赖的信号，以便制定预防进展的策略。抑制含BCR/ABL细胞中ROS产生或阻断Fyn下游信号传导的策略可能提供有希望的治疗方式。

DESCRIPTION (provided by applicant): CML (chronic myeloid leukemia) is unique in that the BCR/ABL oncogene drives disease onset and progression. Therapies inhibiting the BCR/ABL kinase, such as imatinib mesylate, have dramatically altered the treatment of chronic phase disease, but are less effective in blast crisis and against specific point mutations in BCR/ABL. Blast crisis is a very aggressive phase of the disease characterized by genomic instability and secondary mutations. These features have been linked to DNA damage caused by reactive oxygen species (ROS). We and others find that overexpression of p210 BCR/ABL alone causes an increase in levels of ROS. Our preliminary data reveal that a consequence of this increased level of ROS is upregulation of Fyn, a Src kinase family member. We hypothesize that ROS dependent signaling transduced through Fyn contributes to progression to blast crisis. BCR/ABL overexpressing cells display a four-fold upregulation of Fyn protein. This upregulation is blocked by antioxidants, thus linking increased ROS levels and Fyn expression. Point mutations in BCR/ABL that confer resistance to imatinib mesylate retain upregulation of Fyn, suggesting that Fyn may be an appropriate therapeutic target in refractory patients. Knockdown of Fyn using shRNA slows leukemia cell growth in vitro and in vivo, inhibits clonogenic growth by 45% and causes increased sensitivity to imatinib. These striking changes indicate that Fyn is important for leukemia cell proliferation and sensitivity to imatinib. Blockade of Fyn also lowers the overall levels of intracellular peroxides, suggesting that Fyn can amplify ROS levels, thus contributing to subsequent DNA damage and secondary mutations. These preliminary data place Fyn both downstream and upstream of increased ROS, suggesting the presence of a positive feedback loop, whereby Fyn activation through BCR/ABL leads to increased ROS potentially playing an important role in blast crisis. This proposal will elucidate the mechanism(s) and consequences of oxidant-dependent Fyn upregulation and validate these findings in murine models and in clinical specimens. The specific aims of this proposal are to: #1: Determine the mechanism by which BCR/ABL and ROS levels upregulate Fyn. # 2: Evaluate the effects of Fyn upregulation on ROS dependent proliferative signals and DNA damage. #3: Test the relative contributions of BCR/ABL initiated ROS and Fyn upregulation on progression in CML murine models and patient specimens. These data will provide insight into one significant consequence of BCR/ABL-mediated ROS alterations: Fyn upregulation. Our long-term goal is to understand oxidant-dependent signaling in leukemia in order to devise strategies to prevent progression. Tactics that quelch ROS production, or block downstream signaling through Fyn in BCR/ABL containing cells may provide promising therapeutic modalities.